stan bio - uric acid Flashcards
Uricase acts upon uric acid to form ___
hydrogen peroxide and allantoin.
The H2O2
is measured quantitatively by its reaction
with ___
3,5-dichloro-2-hydroxybenzenesulfonic acid (DCHB)
The H2O2
is measured quantitatively by its reaction
with 3,5-dichloro-2-hydroxybenzenesulfonic acid (DCHB)
in the presence of ___
peroxidase and 4-aminophenazone
end product
N-(4-antipyryl)-3-chloro-5-sulfonate-p-benzoquinonemonoimine+ H2O
what are the enzymes used
uricase and peroxidase
Reagent is stable, stored at__ until expiration date on label.
2-8°C
Standard is stable until expiration date on
label when stored at ___
2-8°C
DO NOT FREEZE. Bring reagent to
room temperature before use.
true or false
t
absorbance readings at __ nm
520
Specimen that can be used
Serum, or plasma from heparinized or EDTA blood, is recommended
Urine is diluted 1:10 (1 + 9) with distilled water.
Uric acid in serum, plasma and urine is
reported stable for ___ days at room temperature,
2-3 days
Uric acid in serum, plasma and urine is
reported stable for ___ days at
2-8°C
3-7 days
Uric acid in serum, plasma and urine is
reported stable for __ months when frozen.
6-12 months
