stan bio - uric acid Flashcards
Uricase acts upon uric acid to form ___
hydrogen peroxide and allantoin.
The H2O2
is measured quantitatively by its reaction
with ___
3,5-dichloro-2-hydroxybenzenesulfonic acid (DCHB)
The H2O2
is measured quantitatively by its reaction
with 3,5-dichloro-2-hydroxybenzenesulfonic acid (DCHB)
in the presence of ___
peroxidase and 4-aminophenazone
end product
N-(4-antipyryl)-3-chloro-5-sulfonate-p-benzoquinonemonoimine+ H2O
what are the enzymes used
uricase and peroxidase
Reagent is stable, stored at__ until expiration date on label.
2-8°C
Standard is stable until expiration date on
label when stored at ___
2-8°C
DO NOT FREEZE. Bring reagent to
room temperature before use.
true or false
t
absorbance readings at __ nm
520
Specimen that can be used
Serum, or plasma from heparinized or EDTA blood, is recommended
Urine is diluted 1:10 (1 + 9) with distilled water.
Uric acid in serum, plasma and urine is
reported stable for ___ days at room temperature,
2-3 days
Uric acid in serum, plasma and urine is
reported stable for ___ days at
2-8°C
3-7 days
Uric acid in serum, plasma and urine is
reported stable for __ months when frozen.
6-12 months
Interfering Substances:
Hemoglobin levels greater than ____
bilirubin greater than ___
100 mg/dL,
20mg/dL
standard solution
1000 ul reagent
20 ul standard
sample solution
1000 ul reagent
20 ul sample
time of incubation of cuvert
37°C for 5 minutes and allow to cool, or
incubate at room temperature for 15 minutes
Read S and U vs. RB at 520 nm within ___ minutes.
15
formula
Serum or Plasma Uric Acid (mg/dL) = au/as x 8
urine Uric Acid (mg/dL) = au/as x 80
Normal Range:
Men 3.4 – 7.0 mg/dL
Women 2.4 – 5.7 mg/dl
Urine: 0.5 – 1.0 gram/day
