stan bio - BUN Flashcards
__ is the principle waste product of protein catabolism.
Urea
It is synthesized
in the -__ from ammonia which is produced as a result of the deamination
of amino acids.
liver
It is synthesized
in the liver from ammonia which is produced as a result of the deamination
of ___.
amino acids
Normally, urea nitrogen in the blood comprises only about
___ of the non-protein nitrogen.
45%
. The importance of urea nitrogen determination is its value as an indicator of ____ functions
liver and kidney
Decreases in Blood Urea Nitrogen (BUN) are seen with ___
nephritis,
acute liver destruction,
amyloidosis
and pregnancy
Increases in BUN are encountered with
a
cute and chronic nephritis,
intestinal and urinary obstruction,
uremia,
metallic poisoning,
pneumonia
, Addison’s Disease,
peritonitis,
surgical shock and cardiac failure
Urea is catalytically converted to __ by the
use of urease
ammonium carbonate
The reaction rate is dependent upon the concentration of the
influence of ___.
glutamic dehydrogenase
The rate of this second reaction is
dependent upon the first and can be measured by the rate of conversion of
____ by the change of absorbancy at 340 nm.
NADH to NAD
wavelength
340 nm
enzymes used
urease and glutamic dehydrogenase
end product
L-Glutamate + NAD+ + H2
O
Urea + H2 O —— urease——>
2NH4 + 2HO3
2-Oxoglutarate + NH4 + NADH —- GLDH—-
->
L-Glutamate + NAD+ + H2 O
