stan bio - BUN

Question 1

__ is the principle waste product of protein catabolism.

Answer 1

Urea

Question 2

It is synthesized
in the -__ from ammonia which is produced as a result of the deamination
of amino acids.

Answer 2

liver

Question 3

It is synthesized
in the liver from ammonia which is produced as a result of the deamination
of ___.

Answer 3

amino acids

Question 4

Normally, urea nitrogen in the blood comprises only about
___ of the non-protein nitrogen.

Answer 4

45%

Question 5

. The importance of urea nitrogen determination is its value as an indicator of ____ functions

Answer 5

liver and kidney

Question 6

Decreases in Blood Urea Nitrogen (BUN) are seen with ___

Answer 6

nephritis,
acute liver destruction,
amyloidosis
and pregnancy

Question 7

Increases in BUN are encountered with
a

Answer 7

cute and chronic nephritis,
intestinal and urinary obstruction,
uremia,
metallic poisoning,
pneumonia
, Addison’s Disease,
peritonitis,
surgical shock and cardiac failure

Question 8

Urea is catalytically converted to __ by the
use of urease

Answer 8

ammonium carbonate

Question 9

The reaction rate is dependent upon the concentration of the
influence of ___.

Answer 9

glutamic dehydrogenase

Question 10

The rate of this second reaction is
dependent upon the first and can be measured by the rate of conversion of
____ by the change of absorbancy at 340 nm.

Answer 10

NADH to NAD

Question 11

wavelength

Answer 11

340 nm

Question 12

enzymes used

Answer 12

urease and glutamic dehydrogenase

Question 13

end product

Answer 13

L-Glutamate + NAD+ + H2
O

Question 14

Urea + H2 O —— urease——>

Answer 14

2NH4 + 2HO3

Question 15

2-Oxoglutarate + NH4 + NADH —- GLDH—-
->

Answer 15

L-Glutamate + NAD+ + H2 O

Question 16

Prepare Working Reagent in the ratio of___

Answer 16

5 parts Buffer (R1)
to 1 part Enzyme (R2)

Question 17

Reagents should appear clear and colorless. Discard if either appears __

Answer 17

cloudy or contains particulate matter

Question 18

The Working Reagent is stable for

___ at 2-8°C or
___ at room temperature (15- 25°C).

Answer 18

4 weeks;

5 days

Question 19

Anticoagulants containing __ must be avoided.2

Answer 19

ammonium or fluoride salts

Question 20

Urea in serum is stable for up to __ at room temperature (15-25oC),

Answer 20

24 hours

Question 21

several days refrigerated at ____C

Answer 21

2-8* C

Question 22

at least__ when frozen
(-20oC)

Answer 22

2-3 months

Question 23

interferences

Bilirubin levels up to___,

hemoglobin levels up to

triglyceride levels up to

Answer 23

40 mg/dL

200mg/ dL

2000 mg/dL

Question 24

seconds of reading in BUN

Answer 24

30 and 60 seconds

Question 25

formula for serum bun

Answer 25

Serum BUN (mg/dL) = au/as X 30

Question 26

urea concentration formula

Answer 26

Urea (mg/dL) = BUN (mg/dL) x 2.14

Question 27

Urea (mmol/L) = Urea(mg/dL) x ____

Answer 27

0.167

Question 28

Normal Range:

Answer 28

BUN 8 - 23 mg/dL
Urea 17 - 49 mg/dL