stan bio TPAG Flashcards
Protein molecules contain a large number of __
peptide bonds
When treated with ___in alkaline solution, a
colored complex is formed between the copper and the carbonyl
and imine groups of these peptides
copper ions (Cu+2)
As a similar reaction occurs
with ___ (the simplest of such compounds formed by heating
urea),
the term “___ reaction” was adopted.
biuret
The method biuret reactio described is based on the reports of __
Weischselbaum and Gornal et al
The violet color developed is proportional to the number of ___ in the protein and is nearly independent of the relative concentration of albumin and globulin
peptide bonds
Reagents
Solution of copper sulfate pentahydrate - 0.3 g/dL,
in aqueous sodium hydroxide - 0.8 g/dL
potassium iodide and
potassium sodium tartrate
Total Protein Standard composition
Aqueous solution of Bovine Albumin, Fraction V, with sodium azide as preservative.
____ as preservative.
sodium azide
precaution:
Standard contains sodium azide as a preservative. May react with
____ to form explosive metal azide build up
copper or lead plumbing
Total Protein kit is stable at __ until the expiration date on the label.
2-8°C
Total Protein Reagent is stable at___.
2-30°C
Store standard at__.
2-8°C
Do not use ___, as fibrinogen gives increased results
plasma
Total protein in serum is reportedly stable for
____ at room temperature and for _____ at 2-8°C.
1 week; 30 days
This method is not to be used for the determination of
protein in ____
urine or spinal fluid
This method is not to be used for the determination of
protein in urine or spinal fluid, due to ___
low protein levels and high
concentrations of interfering compounds
Reagent solution
1000 ul of reagent
standard solution
1000 ul of reagent
10 ul of standard
unknown solution
1000 ul of reagent
10 ul of sample
absorbance wavelength
550 nm
. Read S and U vs RB at 550 nm within how many mns or hrs?
within 1 hour
formula
au/as X 10
