stan bio - createnine Flashcards

1
Q

In ___ described a method for the determination of creatinine involving a protein free filtrate and reaction with picric acid
in alkaline solution

A

1886 Jaffe

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2
Q

This reaction is subject to interferences by a number of substances, including ___.

A

protein and glucose

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3
Q

Kinetic procedures
have become popular because they are ____

A

rapid, simple and avoid
interference.

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4
Q

The present method is based on a modification of the
automated reaction rate of ___

A

Fabinay and Eringshausen

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5
Q

Creatinine + Picric Acid =

A

Creatine-picrate complex

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6
Q

wavelenth

A

510 nm

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7
Q

___ to prepare “Working Reagent”.

A

Mix 1 part Creatinine Acid reagent with
1 part Creatinine Base Reagent

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8
Q

Creatinine Acid Reagent, Creatinine Base Reagent and Creatinine Standard are stable until the
date of their expiration when properly stored at ____

A

15-30°C.

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9
Q

Working reagent is stable for 30 days when properly stored at
___

A

15-30°C

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10
Q

do not use Working reagent if turbid.

A

okay puu

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11
Q

Creatinine values have a reported stability of
one day at ___

A

2-8°C

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12
Q

Creatinine values have a reported stability of
one day at 2-8°C, and ____ when frozen (-20°C) and protected from evaporation and contamination.

A

several months

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13
Q

Urine specimens
must be preserved with __

A

15 grams of boric acid

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14
Q

Urine is stable for
___ at room temperature (15-25°C).

A

4 to 7 days

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15
Q

seconds for calculating creatinine

A

20 and 60 secs

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16
Q

formula

A

Serum Creatinine (mg/dL) = au/as X 5

17
Q

Urine Creatinine (formula

A

Urine Creatinine (mg/dL) = au/as x 5 x 20

18
Q

Normal Range:

A

Male (serum): 0.9 - 1.5 mg/dL
Male (urine): 1000 - 2000 mg/24hrs.

Female (serum): 0.7 - 1.4 mg/dL
Female (urine): 600 - 1500 mg/24hrs

19
Q
A