INTRODUCTION, LAB SAFETY AND EQUIPMENT - RODRIGUEZ BASED Flashcards
measurement of analytes may either use the
conventional unit or the systeme international unit
systeme international (SI) unit consist how how many independent base units
7 and each unit represented by a symbol
what are the 7 independent base units under SI units
meter (m)
kilogram (kg)
second -s
mole -mol
ampere - A
Kelvin -K
candela - cd
a newborn infant or neonate is a child under how many days of age
under 28 days of age (WHO)
infants are described of how many yrs of age
0-1 yrs
pediatric is described of how many yrs of age
0-18 yrs (birth to 18 y/o)
blue quadrant in NFPA
health haazard
health haazard criteria
0- normal material
1- slightly hazardous
2- hazardous
3- extreme dange
4- deadly
fire hazard criteria
0 - will not burn
1 - above 200C
2 - below 200C
3 - below 100C
4 - below 73C
reactivity hazard criteria
0 - stable
1 - unstable if heated
2 - violent chemical change
3 - shock and heat may detonate
4 - may detonate
fume hoods must be examined periodically as to ventilation (velocity _____) against inhalation of hazardous and toxic fumes
velocity 100-120 ft/mn
it is recommended that safety showers deliver __ gal. mn with what psi
30-50 gal/mn and psi of 20-50 psi
what are the poisonous vapors listed in rodriguez
chloroform
methanol
carbon tetrachloride
bromide
ammonia
formaldehyde
merycury
what are the human carcinogens listed in the book of rodriguez
BF
benzidine and formaldehyde
what are the probable human carcinogens listed in the book of rodriguez
aniline dyes - (auramine-rhodamine)
if a chemical spills occurs, the first step must be
assist or evacuate the personnel
safety carriers for transport should be provided for bottles of acids and alkalis with volumes greater than ___
500 ml
should we store and arranged chemicals alphabetically?
nope - arrange depending on the quantities of chemicals needed and the nature or types of chemicals
the maximum working volume of flammable solvents allowed outside the storage cabinets is ____ gallon per room
5 gallon
___ must be stored with a provision for second containment because of their tendency to cause damage to the primary compartment
corrosives
some chemicals may deteriorate over time and become hazardous with a tendency to explode such as
ether
peroxides
acids such as acetic acid and sulfuric acid is not compatible with
oxidizing agents such ash
halogens
nitric acid
potassium nitrate
alkali metals such as sodium and potassium is not compatible with
carbon dioxide
chlorinated hydrocarbons
water
bromine and chlorin is not compatible with
acetylene
ammoniahal
halogens are not compatible with
ammonia
acetylene
hydrocarbons
Laboratory-acquired infections are mostly transmitted tru
parenteral inoculations like needlesticks or contaminated sharps, spills and splashes, ingestion, and inhalation
infectious agents that pose the greatest risk are those that are transmitted by __
aerosols
lab procedures that create aerosols
pipetting
flaming looops
agar plates streaking
cebtrifugation
five most common LAIs
shigellosis
salmonellosis
tuberculosis
brucellosis
viral hepatitis
are the recommended measures by the WORLD HEALTH ORGANIZATION to avoid covid 19
handwashing
physical distance
face mask
this type of biosafety cabinet only protects the laboratory worker but no provisions for samples
class I
this class of BSL cabinet allows room (unsterilized air into the cabinet, to circulate around the workspace, and expose the material within, hense samples are exposed to contamination )
class I
this class of BSL cabinet has open fronted cabinet and is only utilized for biological samples
class II type AI
percent of air recirculated and exhausted in class II type A ii and iii cabinet
70% recirculated and 30% exhausted
most commonly used type of BSL cabinet
class II type AII
this BSL cabinet is for both BIOLOGICAL and CLINICAL (infectious) samples and specimens treated with minimal concentration of volatile chemicals
class II type AII
percent of air recirculated and exhausted in class II type B1 cabinet
30% recirculated 70% exhausted
percent of air recirculated and exhausted in class II type B2 cabinet
0% recirculated 100 exhausted
this type of BSL cabinet is for processing chemicals, radioisotopes, and carcinogens aside from biologic samples treated with toxic or hazardous chemicals
clue : radioisotope and carcinogets
class II type BII cabinet
close fronted BSL cabinet with airtight system
class III cabinetc
class III cabinet is also known as
glove-box-cabinet
this BSC has gloves attacged and sealed onto the cabinet
class III BSC
provides the highest level of safety to the lab worker and can handle BSL 4 agents
class III cabinet
this BSC has air coming into and out of the cabinet is sterilized using the HEPA filter
class III
arrange the hierarchy of controls of hazard from most effective to least effective
elimination - most
substitution
engineering controls
administrative control
PPE
is the process where lab practices, control measures, biosafety standards, and the hazardous characteristics of infectious agents are reviewed to prevent lab acquired infections or exposure to high risk pathogen
biorisk assessment
bleach should be in contact with the area for decontamination for how many mns
at least 20 mns
10% solution of common household bleach inactivates hepatitis B virus for how many mns
10 mns
10% solution of common household bleach inactivates HIV virus for how many mns
two minutes
10% solution of common household bleach inactivates hepatitis B virus and HIV for how many mns
HBV - 10 MNS
HIV 2 MNS
the thermometers should be checked periodically against a reference thermometer from the
national institute standard and technology
thermometers that differ by more than ____ from the reference thermometers should be disposed of
1*C
thermometers should be checked daily for the presence of ___- to ensure accuracy of reading
gas bubbles
the speed and revolution per mn of the centrifuge must be checked by
tachometer
what are the equipment that must be test with QC annually
analytical balance
biohazard hood ***
fume hood
what are the equipment that must be test with QC daily
biofreezer and refrigerator
water bath and heating block
what are the equipment that must be test with QC biannual
centrifuge
what are the equipment that must be test with QC every 3-6 months
pipet - single and multi channe;
__ extinguishers are recommended for computers
halogen hydrocarbon extinguishers
the parts of the body most frequently subject to injury in clinical lab are
eyes
mouth
respiratory
GIT
heat is effective for decontamination at what temp and time
250*C for 15 mns
