STAINING Flashcards
(144 cards)
this process renders the different tissue constituents more visible
staining
promotes easier optical differentiation and identification of the cell and tissue components
staining
In general, microscopic examination is facilitated if ___ stains are used
two contrasting
Tissue constituents are demonstrated in sections by direct
interaction
with a dye
or staining solution
, producing
coloration of the active
tissue component
histological staining
types of histological stains
microanatomic stains
bacterial stains
specific tissue stains
specific tissue stains examples
muscles, connective, and neurologic stains
major groups of staining of tissues
histological staining
histochemical staining (histochemistry)
immunohistochemical staining
combination of immunology, histo and chemical staining which uses fluorescent labels
immunohistochemical staining
this group of staining is based on the phenotypic marker
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immunohistochemical staining
is the purified form of a coloring agent or
crude dye that is generally applied in an aqueous solution
histologic stain
- thru chemical reactions that will permit microscopic localization of a SPECIFIC tissue substance
Histochemical Staining (Histochemistry)
example of Histochemical Staining (Histochemistry)
perl’s prussian blue
periodic acid shiff
Perl’s prussian blue is an example of histochemical staining and is used for staining __
hemoglobin
Periodic acid schiff is an example of histochemical stain which is used for ___
staining carbohydrates
a staining procedure wherein the active reagent serves as a substrate upon which the
enzymes act,
enzyme histochemistry
- The process whereby the action of the dye is intensified
by adding another agent or mordant
indirect staining
serves as a
link or bridge between the tissue and the dye, to make the
staining reaction possible
mordant
example of mordants
potassium alum with hematoxylin in ehrlich’s hematoxylin
iron in weigert’s hematoxylin
hematoxylin types depends still on different principle, either __ and ___
regressive and progressive
regressive - uses decolorizer or differentiator
progressive - just do the staining sequence
in enzyme histochemical staining , the final opacity or coloration is produced coming from ____
substrate rather than the tissue
A combination of immunologic and histochemical techniques that allow phenotypic markers to be detected and demonstrated under the microscope.
Immunohistochemical Staining
the process of giving color to the sections by using aqueous or alcoholic dye solutions
direct staining
direct staining examples
methylene blue and eosin
another agent we use in indirect staining which does not participate in the staining but only ACCELERATE
the staining reaction
accentuator