FINALS - MICROTOME, SECTION CUTTING, ADHESIVES, MOUNTING Flashcards
The process by which processed tissue, most commonly a paraffin-embedded tissue, is trimmed and cut into uniformly
thin slices or “sections” to facilitate studies under the
microscope
MICROTOMY
is a skilled process that requires
precision and hand - eye coordination combine
with a delicate touch by the experience
histotechnologist/cian
microtomy
The basic instrument that is capable of cutting a section at a
predetermined thickness by sliding the block into a cutting tool, usually a
steel knife, glass or diamond blade, which is fixed and attached to the
machine.
microtome
3 essential parts of microtome
block holder
knife carrier and knife
pawl, ratchet feed wheel and adjustment screws
microtome essential part where the tissue is held in position
block holder
microtome essential part where its primary purpose is for actual cutting of tissue sections
knife carrier and knife
microtome essential part where its purpose is to line up the tissue block in proper position with the knife, adjusting the proper thickness of the tissue for successive sections
pawl, ratchet feed wheel, and adjustment screws
the wheel that is for forward and backward movement of the tissue block
course wheel
principle of microtomy
a spring-balanced teeth or pawl is brought into contact with, and turns a ratchet feed wheel connected to a
micrometer screw, which is in turn rotated, moving the
tissue block at a predetermined distance towards the
knife for cutting sections at uniform thickness.
what are the kinds of microtome
rocking
rotary
sliding
freezing
cryostat or cold microtome
ultrathin
this kind of microtome is for cutting serial sections of large blocks of paraffin embedded tissues
rocking microtome
this kind of microtome is for cutting paraffin embedded sections
rotary microtome
this kind of microtome is for cutting celloidin embedded sections
sliding microtome
this kind of microtome is for cutting unembedded frozen sections
freezing microtome
microtome for large blocks such as brain, whole organ
rocking microtome
this kind of microtome is for cutting sections for electron microscopy
ultrathin microtome
rocking (cambridge) microtome is invented by whom
paldwell trefall in 1881
is the simplest among the different types of microtomes
rocking (cambridge) microtome
rocking microtome is also called as
cambridge microtome
rocking (cambridge) microtome is consists of a
heavy base
2 arms (the lower arm resting on pivots and a supporting column)
and attached to the micrometer screw at the base of which is found the ratchet wheel with feed mechanism
The Cambridge rocking microtome, available in how many sizes
two
has been used to
cut small and large blocks of paraffin tissues
cambridge rocking microtome
ROCKING (CAMBRIDGE) MICROTOME
a section is thereby cut as the tissue passes to the knife edge in what plane and thickness
slightly curved plane
10-12 u in thickness
rocking microtome where in the block
holder are brought together by __ motions
upward and vertical motions