GROSSING Flashcards
grossing often referred to as
cut up
grossing involves a careful examination and description of the specimen of it’s ____
appearance, number of pieces, and dimensions
the most important processes in which the pathologist arrives at a diagnosis
grossing examination of specimens
things to consider in identification of the specimen
patient’s name and surname, birthday, hospital number.
the container must bear the same name and account number
check the number in the request form
criteria for rejection of the gross specimen
discrepancies between the requisition and specimen label
specimen with no labels or mislabeled
leaking specimen container
absent clinical data or history
inappropriately identified specimen
orientation markers used in describing specimen
inks, nicking, suturing
used to identify and orient the spec component
ink
for indicating laterally
nicking
sutures are represented as
long lateral and short superior
after describing the specimen using orientation marker, what is next
the specimen is then cut into representative sections and put in small plastic cassette to hold the tissue
size of the cassette
3 X 2.5 X 0.4 cm
the specimen should not be more than how many mm in thickness
0.3 mm
purposes of inking
resection margins
embedding instructions
orientation
distinguish between samples
identify the cut surface
acetic acid
responsibility of a technician
specimen preservation
specimen labeling, logging and identification
preparation of the specimen to facilitate their gross and microscopy
record keeping
tissues are classified based on the type of specimen received. for what purpose?
for the purpose of easy identification, ease in handling, and fast retrieving of results in case of future reports are needed
tissue specimens are prepared by the medical technologist for the pathologist to
describe and cut
specimens received in the laboratory, whether tissue or smear, are numbered upon receipt for ease in handling and easy retrieval when further reports or investigations are needed
true or false
true
materials for grossing
tissue specimen
pencil
sharp knife
cutting board
ruler
dental floss
filter paper
scissors
procedure (,manual) of grossubf
cut a 1/2 X 2 inch piece of filter pate
using a pencil, write the number corresponding to the number assigned to you by your instructor
cut a 2.5 X 2 x 0.5 cm tissue
tie the tissue using one end of a dental floss
on the other end of the floss, tie the prepared filter paper containing the specimen number
first and most important step in the pre-analytic phase of histopath section
numbering and accessioning
identify properly all the specimen received without the need of writing the patient’s name to the accompanying specimen tag
numbering and accesioning
in numbering the specimen, numbering is preceded by either
S surgical
A autopsy
C cytology
and year must be indicated
entering details in the log book is part of
numbering and accesioning
recommended steps in tissue grossing
check fixation status
prepare thin slices
avoid specimen trauma
avoid cross contamination
take care with biopsy pads
choose appropriate cassete
avoid overloading cassettes
clearly label cassettes
why do we need to check the fixation status
specimens are dealt with promptly especially large specimens that may otherwise be inadequately fixed
how thin we need to prepare the tissues
3-4 mm maximum thickness. This is particularly important with dense tissues
how do we avoid specimen trauma
handle carefully
do not crush
always use sharp blades
how to avoid cross contamination
each specimen is handled on a clean surface avoiding the possibility of a specimen-to-specimen contamination
what are the specimen that are not placed in biopsy pads
needle core specimens
what are seen in a section that result from local pressure caused by the cellular structure of the foam pads when applied to fresh or briefly fixed tissues
triangular spaces
why do we need to choose appropriate cassettes
tissue fragments shrink during processing, if cassette perforations are too large, fragments may escape into processing reagents, or worse still, transfer over to another specimen
cassettes with fine perforations are available for small tissue fragments
t or f
true
why do we need to prevent overloading cassettes
allowing ready access to processing reagents and preventing distortion of specimens
if the volume of tissue is too great __ is used
second cassette
__ is of paramount importance
accurate identification of specimen
specimen pathology number
accession number
entries in biopsy register
accession number
requisition form as well as the container
accession number
following considerations
check if the specimen is properly labeled with name, age, hospital registration no
the nature of tissue to be exami8ned and the requisition form is also duly filled
check if the specimen is in proper fixative
