LEC6 - INTRO TO HISTOPATHOLOGIC TECHNIQUES Flashcards
is defined as the process of preparing the tissue
tissue processing
refers to a better and more effective means of studying tissues whether normal or abnormal is by examination of their sections and smears which have been permanently preserved, stained, and mounted on glass slides with cover slips for permanent keeping
- Processing of Tissue
- Why examine histopathologic specimens?
- Pathology attempts to explain the WHYs and wherefores of the signs and symptoms manifested by patients while providing a sound foundation for rational clinical care and therapy.
- Determine if the sample is benign or malignant
is the microscopic study of the normal tissues of the body
histology
is the microscopic study of tissues affected by disease
histopathology
procedures adopted for the preparation of material for such studies
histologic or histopathologic technique
the examination of tissue has 2 major type of tissue
fresh and preserved
tissue processing is a process, by means of process, it refers to the phases which are
pre analytic
analytic
post analytic
the method of tissue examination may vary according to the following
the structural and chemical components of the cells to be studied
the nature and amount of the tissue to be evaluated
2 types of tissue to be examined
fresh and frozen tissue
- ___ are usually examined when there is an immediate need for evaluation
Fresh Tissues
- Advantages of fresh tissues
o Examined in the living state thereby allowing protoplasmic activities such as: motion, mitosis, phagocytosis and pinocytosis to be observed
again, what are the protoplasmic activities we can observed if we are using fresh tissues
motion, mitosis, phagocytosis, and pinocytosis
- Disadvantages of the use of fresh tissues
its use has been limited/ the protoplasmic activities are limited in time only
liable to develop the changes that have been usually been observed after death
advantage of preserve tissue
once the cellular activity of tissue has stopped is preserved in life-like manner
METHODS OF FRESH TISSUE EXAMINATION
● Teasing or Dissociation
● Squash Preparation (Crushing)
● Smear Preparation
○ Streaking
○ Spreading
○ Pull-apart
○ Touch preparation (Impression smear)
● Frozen Section
A method in fresh tissue examination whereby a selected tissue specimen is immersed in a watch glass containing isotonic salt solution
Teasing or Dissociation
hypertonic solution can cause the cell to
shrink
hypotonic solution can cause the cell to
swell
teasing or dissociation method of fresh tissue is examined using what microscopy
unstained and examined using phase contrast and bright field microscope
if we want to stain the specimen by examining it using teasing or dissociation method, we can use ____ dye
methylene blue dye
CRUSHING method is also called as
squashing preparation
a process whereby a small piece of tissue in is placed in microscopic slide and forcible compressed with another slide or with a cover glass
squash preparation (crushing)
how many diameter is the limit for squash preparation
not more than 1 mm