LAB1 - Laboratory Safety, Instrumentation, and Quality Management System in Histopathologic Laboratory Flashcards
Art of analyzing and interpreting the shapes, sizes and
architectural patterns of cells and tissues within a given
specific clinical background
Histopathology
3 phases or activities in histopathology
o Pre-analytical
o Analytical
o Post-analytical
The art and science performed by the histotechnologist to
produce a tissue section of good quality
Histotechnology
responsible for the processing of tissue
sections
Histotechnologist
Provide the basic concepts about the principles and
technicalities involved in histopathologic procedures
Histopathologic Techniques
Provide skills in tissue preparation from fresh to properly
mounted specimen
Histopathologic Techniques
what is the purpose of fixation
to preserve the cellular component of the cells and the tissue particularly the nuclear and cytoplasm
Is comprised of a number of glass beakers for filling different
processing chemicals
Automatic Tissue Processor/ Autotechnicon
how many glass beakers are in Autotechnicon
12 beakers
what are the processes that Autotechnicon can handle?
fixation
dehydration
clearing
infiltration
the product of Automatic Tissue Processor is called as
autotechnicon
what are the 11 processes of histopathologic techniques
fixation
***Decalcification(optional)
dehydration
clearing
infiltration
embedding
trimming
sectioning
staining
mounting
labelling
what is the ideal fixative
formalin 10%
ideal agent for dehydration
ethanol - 70% to absolute acetic concentration
ideal clearing agent
xylene
medium used in infiltration
parafilm
medium used in embedding
parafilm, same with infiltration
) a ____ is used for the sectioning of the tissue
microtome
process of cutting into thin sections
Microtomy
An instrument where tissues are cut into sections
Microtome
Embedding media:
paraffin wax
thin sections of tissue are called as
Paraffin ribbons
what is the instrument that is embedded in cryostat
rotary microtome
Used when the specimen is
subjected for the frozen section
Cryostat