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PH 166 > Specimen Collection &Handling > Flashcards

Specimen Collection &Handling Flashcards

1
Q

Which may directly affect hormone level in blood?

A

Tourniquet application

Diurnal variation

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2
Q

Change in posture may elevate __&__levels

A

Albumin

Calcium

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3
Q

Which may be affected by stress? __ increased & __ decreased

A

Total cholesterol

HDL cholesterol

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4
Q

What indicators may be increased due to fasting for both sexes after 48 hr?

A

Serum bilirubin

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5
Q

What indicators may be decreased due to fasting in women, after 72 hr?

A

Plasma glucose

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6
Q

Tourniquet application –> increased enzyme and protein bc __

A

Hemoconcentration

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7
Q

Most serum constituents remain constant in adults until __ in women & __in men

A

Menopause

Middle age

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8
Q

Which age group has low glycogen reserve?
A. Infant
B. Adult
C. Same

A

A

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9
Q

Enumerate physio factors that may contribute to result variation

A 
Stress
Diurnal Variation
Diet
Exercise
Age
Torniquet Application
Posture
Gender
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10
Q 
Tobacco smoking is an in-vivo interference to the lab result. It may elevate all except:
A. Hb conc
B. HDL
C. MCV
D. RBC count
E. WBC Count
A

B

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11
Q 
When the needle is \_\_\_, hemolysis may occur
A. too small
B. too big
C. either A or B
D. neither A nor B
A

A

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12
Q

Serum bilirubin > 430 mmol/L is called ___

A

icteric serum

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13
Q 
icteric serum: interference for assays of all except
A. albumin
B. total protein
C. cholesterol
D. bilirubin
A

D

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14
Q 
lipemic serum: interference for assays of all except
A. urea
B. total protein
C. cholesterol
D. bilirubin
A

C

amylase dapat

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15
Q

Arterial puncture: used for all except
A. complete blood count
B. pH measurement
C. blood gas analysis

A

A

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16
Q 
Unacceptable sites for arterial puncture include all except
A. inflammation site
B. site near a wound
C. site with AV fistula
D. site with deep arteries
A

D

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17
Q

identify most common preanalytic error in blood gas measurement

A

use of too much heparin

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18
Q 
all are common sites for venipuncture except
A. antecubital fossa
B. wrist and ankle
C. hand dorsum
D. jugular vein
A

D

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19
Q

antecubital fossa includes these 3 veins. enumerate

A

median cubital vein
cephalic vein
basilic vein

20
Q

purpose of Heel Skin/Finger Puncture

A

routine assays requiring small amounts of blood

21
Q

order of draw: evacuated tube and syringe

A
  1. yellow
  2. blue
  3. serum tubes with/without clot activ/gel sep
  4. green
  5. lavender
  6. silver
22
Q 
additive || color top
Which is matched properly?
A. EDTA || red
B. sodium heparin || gray
C. sodium fluoride || lavender
D. sodium citrate || blue
A

D

A. no additive || red
B. sodium heparin || green
C. sodium fluoride || gray

23
Q 
additive || color top
Which is matched properly?
A. EDTA || lavender
B. SST || blue
C. sodium heparin || gold
D. sodium citrate || yellow
A

A

B. SST || gold
C. sodium heparin || green
D. sodium citrate || blue

24
Q 
additive || color top
Which is matched properly?
A. SST || silver
B. sodium fluoride || gold
C. sodium heparin || green
D. sodium citrate || lavender
A

C

A. SST || gold
B. sodium fluoride || gray
D. sodium citrate || blue

25
Q

concentration of EDTA @ tube

A

1-2 mg/mL of blood

26
Q

lavender EDTA tube: purpose

A

hematology cell count and cell morphology

27
Q

pink EDTA tube: purpose

A

ABO grouping, Rh typing, antibody screening

28
Q

white EDTA tube: purpose

A

molecular diagnostic testing for plasma

29
Q

sodium citrate black tube: purpose

A

Westergren sedimentation rate

30
Q

sodium citrate light blue tube:purpose

A

coagulation testing

31
Q

sodium citrate : concentation @ tube

A

3.2-3.8 g/dL of blood

32
Q 
true about heparin
A. used in hematology testing
B. affect ion levels, like calcium
C. can interfere with some immunoassays
D. NOTA
A

C

33
Q

Differentiate lithium heparin fr sodium heparin

A

LiHep - used for most chemistry tests except lithium and folate
NaHep - anticoagulant therapy, trace elements, toxicology

34
Q 
sodium fluoride: gray top. Which is false?
A. used for measurement of heavy metals
B. interferes with Na, K
C. forms weakly dissoc Ca cpds
D. NOTA
A

A

glucose dapat

35
Q

sodium fluoride : concentation @ tube

A

10 mg/mL of blood

36
Q

oxalate : concentation @ tube

A

1-2 mg/mL of blood

37
Q

Why can’t oxalate be used in hematocrit?

A

It may shrink red cells

38
Q

What may be in SSTs? Enumerate

A

clot activator

gel separator

39
Q

red tube: no additive. three purposes?

A

blood bank
immunology assays
blood culture

40
Q

enumerate 5 process that affect concentration of a blood constituent in the specimen during storage

A
  1. adsorption to glass or plastic tubes
  2. protein denaturation
  3. evaporation of volatile compounds
  4. water mvmt into cells
  5. cont metab activities of RBC, WBC
41
Q 
These specimens must be kept  at 4  deg C immediately after collection and transported on ice. Applicable to the ff except
A. NH3
B. plasma renin
C. acid phosphatase
D. glucose
A

D

42
Q

Serum required for the ff except
A. protein electrophoresis
B. coagulation measurements
C. immunofixation assay

A

B

43
Q

adequate time for clotting must be allowed before centrifugation. why?

A

prevent latent fibrin formation, which may cause undesirable clogging of automated chemistry analyzers

44
Q

Three possible events in blood sample that may affect spectrophotometric methods of analysis. enumerate

A

hemolysis
icterus
lipemia

45
Q

icterus may affect spectrophotometric method result because of ___, which has high absorbance at 340-500 nm

A

bilirubin

46
Q

refers to the exclusion of water and small molecules in the aqueous phase, when more volume within a specimen is occupied by protein or lipid that excludes water

A

Solvent Exclusion Effect

PH 166 (28 decks)

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