Lab - Bilirubin, AST, ALT Flashcards
Bilirubin reacts with diazotized sulphanilic acid (DSA) to form a diazo dye. What color?
A. red
B. blue
C. green
A
Which is false?
A. Water-soluble bilirubin glucoronides react directly with diazotized sulphanilic acid
B. Sulphanilic acid & sodium nitrite form diazotized sulphanilic acid
C .Bilirubin + DSA —> Total Azobilirubin
(C - Direct kasi dapat! pag may accelerator, total na.)
[Serum Bilirubin] Absorbance of the diazo dye at \_\_\_is directly proportional to bilirubin concentration in sample A. 345 nm B. 546 nm C. 467 nm
B
Differentiate the procedure of measuring total bilirubin fr measuring direct bilirubin.
time of incubation! direct bil - exactly 5 min. total bil - 10 to 30 min
A.Reference range of total bilirubin in adults(mg/dL): ___
B.Reference range of direct bilirubin in adults(mg/dL): __
A. 1.1 mg/dL
B. 0.25 mg/dL
Which is false?
A. It is important to ensure the bilirubin reagent and the nitrite reagent are thoroughly mixed before adding the sample.
B. Bilirubin levels may be increased if the samples are exposed to light.
C. Hemolysis will lower the bilirubin value due to the inhibitory effect of hemoglobin on the diazo reaction.
B - decreased dapat
[ALT]
Alanine aminotransferase catalyzes the reaction between 2-oxoglutarate and L-alanine. Specify the products that are formed
L-glutamate and pyruvate
[ALT] Pyruvate reacts with \_\_ and hydrogen ion to form \_\_ & lactate A. FADH2 --> FADH B. FADH --> FADH2 C. NADH --> NAD+ D. NAD+ --> NADH
C
[ALT] ALT conc is measured spectrophotometrically. It is measured in any of the ff wavelengths except: A. 365 nm B. 340 nm C. 334 nm D. 321 nm
D
[ALT] False about reference range of ALT: A. Men have bigger amount than women B. Women have bigger amount than men C. It depends on the temperature
A
[ALT]
specimen for testing
blood serum
[AST]
Aspartate aminotransferase catalyzes a reaction that yields L-glutamate & oxaloacetate.
What are the reactants?
2-oxoglutarate + L-aspartate
[AST]
When oxaloacetate reacts with NADH and Hydrogen ion, what is formed aside from NAD+?
L-malate
[ALT/AST]
difference of TRIS buffers?
pH! for ALT: pH 7.5 ; for AST: pH 7.8
[AST]
False about AST reference range
A. women have higher reference value range than men
B. men have higher reference value range than women
C. it depends on the temperature
B