Lab - ALP, ACP Det

Question 1

A.At what wavelength do you measure alkaline phosphatase conc?
B. Increase of absorbance at ___ (wavelength) proportional to total ACP activity in sample

Answer 1

A and B = 405 nm

Question 2

1-Naphthyl phosphate is hydrolyzed by ___ to phosphate and 1-naphthol, which is converted with FRTR-salt to an azo dye.

A.amylase
B.alkaline phosphatase
C.acid phosphatase

Answer 2

C.acid phosphatase

Question 3

p-Nitrophenylphosphate + H2O –> phosphate + p-nitrophenol

What enzyme catalyzes this reaction?
A.amylase
B.alkaline phosphatase
C.acid phosphatase
D.AOTA

Answer 3

B

Question 4

Not a part of Reagent 1 @ alkaline phosphatase test
A. diethanolamine buffer
B. magnesium chloride
C. p-Nitrophenylphosphate
D. sodium azide

Answer 4

(C! nasa reagent 2 siya :D.

Note: sodium azide is the preservative)

Question 5

Which holds true for ALP determination?
A. Mix, read the absorbance after 1 min and at the same time start the stopwatch. Read the absorbance again exactly after 1, 2 and 3 mins.
B. Mix, read the absorbance after 5 min and at the same time start the stopwatch. Read the absorbance again exactly after 5, 10 and 15 mins.
C. Depends on temperature of the room
D. NOTA

Answer 5

A

Question 6

Which is true?
A. Women always have higher levels for ALP reference
B. Men always have higher levels for ALP reference
C. P-nitrophenol is poisonous when inhaled, swallowed or when absorbed through the skin.
D. There is one point when men & women have equal levels for ALP ref

Answer 6

D. this is at 37 deg C = 64-306 U/L

Question 7

A. How many reagents are there in the acid phosphatase test?
B. Prostatic ACP can be blocked by __ and can be determined indirectl by calculating activity diff
C. most common specimen used for ACP det

Answer 7

A. 3
B. tartrate
C. serum

Question 8

Enumerate ACP test reagents + respective components of each rgt

Answer 8

1. buffer - citrate
2. stabilizer - acetic acid
3. substrate - 1-Naphthyl phosphate & Fast Red TR-salt

Question 9

True about ACP det
A. There is only one absorbance reading
B. First absorbance read after 3 min, second one after another 3 min (30 deg C)
C. First absorbance read after 5 min, second one after another 5 min (25 deg C)

Answer 9

(C)

Question 10

True about ACP reference range
A. Men have higher ACP range than women
B. Women have higher ACP range than men
C. depends on temperature

Answer 10

(A)

Question 11

Reference Range for ACP Det @ 25 deg C: which?
A. female: ≤ 5.5 U/L, male: ≤ 6.5 U/L
B. female: ≤ 4.2 U/L, male: ≤ 5.0 U/L
C. female: ≤ 3.0 U/L, male: ≤ 3.0 U/L

Answer 11

C

A - 37 deg C ; B - 30 deg C

Question 12

Reference Range for ALP det @ 25 deg C: which?
A. male: 49-232 U/L, female: 61-232 U/L
B. male: 40-190 U/L, female: 50-190 U/L
C. female: 49-232 U/L, male: 61-232 U/L
D. female: 40-190 U/L, male: 50-190 U/L

Answer 12

B