Lab - Salicylates, Chloride, Amylase Flashcards
[Serum Amylase]
Which is false?
A.The enzyme in a fixed amount of serum is allowed to act on a fixed amount of starch under fixed conditions.
B. The amount of starch destroyed is measured by adding iodine and comparing the amount of color produced with that of a solution with I but not AMY.
C. End color: green
C - blue, not green
[Serum Amylase] Which isn't a reagent in this test? A.0.85% NaCl B.phosphate buffer C.stock & working iodine solutions D.starch solution E.stock amylase
E
Which is false?
A.1 unit amylase = amount of enzyme needed to hydrolyze 10 mg starch in 30 minutes
B.1 unit amylase = amount of enzyme needed to hydrolyze 20 mg starch in 40 minutes
C.2 unit amylase = amount of enzyme needed to hydrolyze 10 mg starch in 5 minutes
D.NOTA
A
Wavelength range @ serum amylase test: A.550-700 nm B.660-800 nm C.450-750 nm D.NOTA
B
Reference range for serum amylase:
A.15-90 U/L
B.20-110 U/L
C.25-130 U/L
C
Serum Chloride Determination is also called: \_\_ A.Scales & Scales Method B.Shales & Shales Method C.Schales & Schales Method D.Sales & Sales Method
C.Schales & Schales Method
titrant in serum chloride det A. standard mercuric nitrate B. standard mercuric nitrite C. standard mercurous nitrite D. standard mercurous nitrate
A
indicator @ serum chloride det A. s-diphenylnitrazone B. s-diphenylcarbazone C. s-triphenylchlorazone D. s-tetraphenylquinazole
B
End color of complex @ serum chloride det A.tangerine B.auburn C.cyan D.lavender
D
not a reagent in testing serum chloride A. H2SO4 B. HNO3 C. NaOH D. NaCl
(C - sa creatinine ito)
What kind of protein-free filtrate must be prepared @ serum chloride det?
A.Folin-Wu
B.Somogyi
C.Any of the two
A
Reference range of chloride @ serum plasma A. 87-100 mmol/L B. 95-108 mmol/L C. 86-105 mmol/L D. 98-107 mmol/L
D
Reference range of chloride @ 24hr urine A. 2-17 mmol/L B. 1-25 mmol/L C. 20-175 mmol/L D. 11-250 mmol/L
D
main reagent for testing presence of salicylates in serum A.Triner's reagent B.Tinder's reagent C.Trinder's reagent D.Tiner's reagent
C
[Salicylates] wavelength for testing supernatant? A.360 nm B.450 nm C.540 nm D.630 nm
C
[Salicylates]
Which is false?
A.The serum has to be centrifuged before applying reagent
B.The reagent has to be mixed with serum before centrifugation
C. Either is fine
D. It depends on the temperature
(B – after centrifugation, supernatant is subjected to spectrophotometry)
[Salicylates] What is the main testing reagent composed of? A.ferric nitrate and mercuric chloride B.ferrous nitrite and mercuric chlorate C.ferric nitrite and mercurous chloride D.ferrous nitrate and mercurous chlorate
A
Serum salicylate conc above \_\_\_ --> evidence of toxicity (tinnitus) A.10 mg/dL B.20 mg/dL C.30 mg/dL D.40 mg/dL
C
\_\_\_ is much more likely to contain interferences for salicylate det (i.e. phenothiazines) A.serum of blood B.urine C.BOTA D.NOTA
B
