Lab - Salicylates, Chloride, Amylase

Question 1

[Serum Amylase]
Which is false?
A.The enzyme in a fixed amount of serum is allowed to act on a fixed amount of starch under fixed conditions.
B. The amount of starch destroyed is measured by adding iodine and comparing the amount of color produced with that of a solution with I but not AMY.
C. End color: green

Answer 1

C - blue, not green

Question 2

[Serum Amylase]
Which isn't a reagent in this test?
A.0.85% NaCl
B.phosphate buffer
C.stock & working iodine solutions
D.starch solution
E.stock amylase

Answer 2

E

Question 3

Which is false?
A.1 unit amylase = amount of enzyme needed to hydrolyze 10 mg starch in 30 minutes
B.1 unit amylase = amount of enzyme needed to hydrolyze 20 mg starch in 40 minutes
C.2 unit amylase = amount of enzyme needed to hydrolyze 10 mg starch in 5 minutes
D.NOTA

Answer 3

A

Question 4

Wavelength range @ serum amylase test:
A.550-700 nm
B.660-800 nm
C.450-750 nm
D.NOTA

Answer 4

B

Question 5

Reference range for serum amylase:
A.15-90 U/L
B.20-110 U/L
C.25-130 U/L

Answer 5

C

Question 6

Serum Chloride Determination is also called: \_\_
A.Scales & Scales Method
B.Shales & Shales Method
C.Schales & Schales Method
D.Sales & Sales Method

Answer 6

C.Schales & Schales Method

Question 7

titrant in serum chloride det
A. standard mercuric nitrate
B. standard mercuric nitrite
C. standard mercurous nitrite
D. standard mercurous nitrate

Answer 7

A

Question 8

indicator @ serum chloride det
A. s-diphenylnitrazone
B. s-diphenylcarbazone
C. s-triphenylchlorazone
D. s-tetraphenylquinazole

Answer 8

B

Question 9

End color of complex @ serum chloride det
A.tangerine
B.auburn
C.cyan
D.lavender

Answer 9

D

Question 10

not a reagent in testing serum chloride
A. H2SO4
B. HNO3
C. NaOH
D. NaCl

Answer 10

(C - sa creatinine ito)

Question 11

What kind of protein-free filtrate must be prepared @ serum chloride det?
A.Folin-Wu
B.Somogyi
C.Any of the two

Answer 11

A

Question 12

Reference range of chloride @ serum plasma
A. 87-100 mmol/L
B. 95-108 mmol/L
C. 86-105 mmol/L
D. 98-107 mmol/L

Answer 12

D

Question 13

Reference range of chloride @ 24hr urine
A. 2-17 mmol/L
B. 1-25 mmol/L
C. 20-175 mmol/L
D. 11-250 mmol/L

Answer 13

D

Question 14

main reagent for testing presence of salicylates in serum
A.Triner's reagent
B.Tinder's reagent
C.Trinder's reagent
D.Tiner's reagent

Answer 14

C

Question 15

[Salicylates]
wavelength for testing supernatant?
A.360 nm
B.450 nm
C.540 nm
D.630 nm

Answer 15

C

Question 16

[Salicylates]
Which is false?
A.The serum has to be centrifuged before applying reagent
B.The reagent has to be mixed with serum before centrifugation
C. Either is fine
D. It depends on the temperature

Answer 16

(B – after centrifugation, supernatant is subjected to spectrophotometry)

Question 17

[Salicylates]
What is the main testing reagent composed of?
A.ferric nitrate  and mercuric chloride
B.ferrous nitrite and mercuric chlorate
C.ferric nitrite and mercurous chloride
D.ferrous nitrate and mercurous chlorate

Answer 17

A

Question 18

Serum salicylate conc above \_\_\_ --> evidence of toxicity
(tinnitus)
A.10 mg/dL
B.20 mg/dL
C.30 mg/dL
D.40 mg/dL

Answer 18

C

Question 19

\_\_\_ is much more likely to contain interferences for salicylate det (i.e. phenothiazines)
A.serum of blood
B.urine
C.BOTA
D.NOTA

Answer 19

B