Lab - Hc, Hb, Porphy Det

Question 1

Identify the ff:
A.proportion of the volume of red cells to the volume of whole blood
B.in testing A, this is used to seal the lower end of the capillary tube

Answer 1

A.hematocrit

B.plastecin/modeling clay

Question 2

Hematocrit = L1/L2
A. what is L1
B. what is L2
C. what is the ref range

Answer 2

A. L1 is the height of red cell column in mm

B. L2 is the height of the whole blood specimen in mm C. Reference values: 35-47%

Question 3

This solution turns hemoglobin to methemoglobin to cyanmethemoglobin. What is this solution?

Answer 3

Drabkin’s solution

Question 4

not part of Drabkin's solution composition
A.sodium bicarbonate
B.sodium thiosulfate
C.potassium cyanide
D.potassium ferricyanide

Answer 4

B

Question 5

Which is not true about Drabkin’s solution?
A. Salts and solution of cyanide are poisonous, care should be taken while handling such salts and solution
B. Drabkin’s solution is not sensitive to light.

Answer 5

B. it should be stored in a brown bottle in the dark

Question 6

Which is true about hemoglobin?
A.Hgb (g/dL) = 3.68 x A
B.Reference values for male: 12-16 g/dL
C.Reference values for female:14-18 g/dL

Answer 6

A

Question 7

identify:
A. metabolic intermediates in the biosynthetic pathway
that has heme as its principal product
B. inherited and acquired disorders characterized by aberrations in the activities of specific enzymes of the heme biosynthetic pathway

Answer 7

A. Porphyrins

B. Porphyrias

Question 8

A. Identify:red fluorescence exhibited by all the porphyrins when irradiated with light at a wavelength near 400 nm;

B. approp qty meas of porphyrins are based on preliminary extraction and differentiation by solvent partition followed by what techniques?

Answer 8

A.Soret band

B.spectrophotometric or fluorometric measurement

Question 9

Which is false?
A. Lead has effects on heme synthesis
B. Lead toxicity may be manifested by decrease in
erythrocyte protoporphyrin (EP)
C. NOTA

Answer 9

B kasi increase dapat

Question 10

not a reagent in porphyrin determination
A.methyl acetate 
B.Formic acid
C.1.5 N HCl
D.Diethyl ether

Answer 10

A

ethyl dapat

Question 11

identify ref values for:
A. Erythrocyte Coproporphyrin
B. Protoporphyrin

Answer 11

A. Erythrocyte Coproporphyrin: 0.5-2.0 ug/dL (0.75-3.0 nmol/L)
B. Protoporphyrin: 4.0-52.0 ug/dL (7.2- 93.6 nmol/L)