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PH 166 > PH166 intro/Analytical Components > Flashcards

PH166 intro/Analytical Components Flashcards

1
Q

List grades of water @ clin lab

A

Feedwater/tap water

Clinical laboratory reagent water

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2
Q

Clinical laboratory reagent water - how is it prepared? What methods?

A

Filtration
Ion exchange
Carbon adsorption chromatography
UV light treatment

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3
Q

quality control and impurity testing procedures used for diff grades of reagent water - enumerate

A

Microbial monitoring
Resistivity measurements
Silica measurements
Biannual decontamination

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4
Q

Differentiate between a primary standard and Standard Reference Material

A

SRMs are preparation of stated composition that may be used as primary standards

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5
Q

Differentiate bet “to contain” & “to deliver”

A

“To contain” - to hold but not transfer

“To deliver” - transfer

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6
Q

Explain difference bet volumetric pipets and graduated pipets

A

Volumetric - designed to deliver a fixed volume without further subdivisions

Graduated - capable of dispensing several diff volumes

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7
Q

Safety regulations of the OSHA require two things: enumerate

A

Chemical Hygiene Plan

Fire Safety Plan

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8
Q

Define MSDS and explain its use

A

Contains info abt physical & health hazards for all chemical, reagents and kits used by laboratory

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9
Q

Meaning of National Fire Protection Agency blue diamond?

A

Health hazard

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10
Q

Define a desiccant and explain how it is used

A

Desiccant are chemicals that usually are found in a solid form & can absorb water. It may be used to dry and keep dry another chemical

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11
Q

Grades of Chemicals used: enumerate, and rank accdg to quality

A
  1. Analytical grade
  2. Chemically pure grade
  3. US Pharmacopeia/National Formulary
  4. Technical/Commercial Grade
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12
Q

highly purified chemical that can be measured directly to produce a substance of exact known concentration and purity

A

Primary Standard

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13
Q

T/F

Not all standar reference materials are as pure as primary standards

A

T

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14
Q

Organic solvents that are spectrograde/nanograde/HPLC Grade must meet some criteria. enumerate :)

A
  1. purity ensures minimal spectral interference and min residual conc
  2. > 99% pure. no single impurity exceeding 0.2%
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15
Q

What is cobalt chloride’s role in a desiccant?

A

moisture-sensitive indicator salt present in most desiccants. indicates exhaustion

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16
Q

False about anhydrous calcium chloride
A. high capacity
B. works well in

A

C

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17
Q 
True for both anhydrous MgSO4 & anhydrous CaSO4
A. inexpensive
B. fast acting
C. both A and B
D. neither A nor B
A

B

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18
Q

Why can’t Type I reagent water be absorbed for a long time?

A

it may absorb carbon dioxide

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19
Q

used in preparing buffers and standard solutions
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

A

A

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20
Q

used in quali chem, hema, microbio procedures
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

21
Q

used in general urinalysis
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

22
Q

T/F

Sterile water is always reagent water in hospitals

A

F

not always

23
Q

T/F

As water purity increases, water conductivity increases.

A

F

water purity–> less dissolved ionized substance –> less conductivity –> more resistance

24
Q

False about distillation
A. removes volatile impurities
B. meets specs for type II & III water
C. not useful for elimination of low-boiling org cpd
D. removes Fe, Mg, Ca + microbes

25
Q

False about deionization
A. water passed thru resin column
B. (+) and (-) charged particles in column combine with ions present to in water to remove them
C. substances that don’t ionize are removed

A

C

They are not removed

26
Q

A deionization process is source of type I water if further treated with __ & __

A

membrane filtration

activated charcoal

27
Q

False about water purification?
A. ozone: used in industrial setting
B. reverse osmosis: removes dissolved gases effectively
C. UV oxidation- removes bacteria and org cpds

A

B

it does not remove such gases effectively

28
Q

Which is false about tubing?
A. latex rubber tubing: not affected by oils
B. neoprene: not used with chlorinated HCs
C. Tygon tubing:inert to chemicals
D. Teflon tubing: more expensive than Tygon

29
Q

Pyrex and Kimax: type of __ glass

A

borosilicate

30
Q

glassware: ideal for ___ solution

Acidic, alkaline

31
Q

plasticware: ideal for ___ solution

Acidic, alkaline

32
Q

Which is more apt for use @ high thermal experiments?
A. glassware
B. plastic ware
C. either

33
Q

Which is more apt for use @ cryogenic experiments?
A. glassware
B. plastic ware
C. either

34
Q

Differentiate Mohr pipet fr Serological pipet

A

Mohr - calibrated between two marks of the stem. may space after ng dulo ng marks
Serological - has graduation marks down on the tip

35
Q

pipet with bulb closer to delivery tip. used for measuring viscous fluid in blood/serum

identify

A

Ostwald-Folin pipet

36
Q

Mohr pipet or Serological pipet

Which has larger orifice?

A

Serological pipet

37
Q

Which micropipets may also be used to deliver substances?

A

Lang-Levy

Sanz

38
Q 
Conditions in cleaning glassware & reagent
 which is mismatched?
A. blood clot: 2-4% cresol solution
B. metal ion det: 20% nitric acid
C. new pipets: 5% HCl
A

A

10% NaOH dapat

39
Q

Conditions in cleaning glassware & reagent
which is mismatched?
A. bacteriologic glassware: 2-4% cresol solution
B. iron determination: 1:3 nitric acid
C. permanganate stains: 5% HCl

A

C

50% dapat

40
Q

differentiate flammable fr combustible liquid

A

flammable may form ignitible mixture @ 37.8 deg C

41
Q

T/F
Non-ionizing radiation equipment often emits a variety of wavelengths of EM radiation that must be protected against, with the use of PPE

42
Q

In decontamination, identify 2 common solutions used

A

sodium hypochlorite

70% alcohol

43
Q

Meaning of National Fire Protection Agency red diamond?

A

flammability

44
Q

Meaning of National Fire Protection Agency yellow diamond?

A

reactivity warning

45
Q

Meaning of National Fire Protection Agency white diamond?

A

special hazard info

46
Q

Enumerate elements of Hazard Warning System @ reagent bottle.

A 
1- identify hazardous chemical
2- route of body entry
3- health hazard
4- physical hazard
5- target organ affected
47
Q

Which is falsE?
A. solvents such as xylene and acetone may not be redistilled anymore
B. flammables can be incinerated with afterburners & scrubbers to remove toxic products of combustion
C. both A and B
D. NOTA

48
Q

Enumerate approved methods for treating biomedical waste.

A

1- incinerator
2- steam sterilization
3- chemical disinfection
4- encapsulation @ solid matrix

PH 166 (28 decks)

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