Special culture requirements

Question 1

Media used for isolation

chocolate agar with factors V (NAD+) and X (Hematin)

Answer 1

H. influenzae

Question 2

Media used for isolation

thayer-Martin (VPN) media- Vancoymcin (inhibits G+ organisms), Polymyxin (inhibits G- organisms excepted Neisseria) and Nystatin (inhibits fungi)

VPN

Answer 2

• N. gonorrhoeae*
• N. meningitidis*

Question 3

Media used for isolation

Bordet-Gengou (potato) agar,

Answer 3

Bordetella pertussis

Question 4

Media used for isolation

Tellurite agar, Loffler medium

Answer 4

C. diphtheriae

Question 5

Media used for isolation

Lowenstein-Jensen agar

Answer 5

M. Tuberculosis

Question 6

Media used for isolation

Eaton agar, requires cholesterol

Answer 6

M. Pnemoniae

Question 7

Media used for isolation

Pink colonies on MacConkey agar (fermentation produces acid, turning colony pink)

*E. Coli *is also grown on eosin-Methylene blue (EMB) agar as colonies with green metallic sheen

Answer 7

Lactose-fermenting enterics.

Question 8

Media used for isolation

Charcoal yeast extract agar buffered with cysteine and iron

Answer 8

Legionella

Question 9

Media used for isolation

Sabouraud agar

‘Sab’s a fun guy’

Answer 9

Fungi

Used to allow only the growth of fungi and nothing else on the agar.