SB1h - Enzyme Activity Flashcards
What can be combined to produce a graph on enzyme activity?
measure the time taken for an enzyme to complete the breakdown of a substrate at different temperatures
How can the graph showing the time at temperatures show the rate of reaction?
By calculating the amount of substrate broken down or product formed in a given time
100g of starch was broken down in 5 minutes, what was the mean reaction rate?
100/5 = 20g/min
What happens when temperature increases?
The reaction gets faster
Why does the reaction get faster?
As the temp increases, the enzyme and the substrate get faster leading to more collisions per second.
What’s the optimum temperature?
When the enzymes working at its fastest possible rate
What happens when the temperature increases past the optimum?
Enzyme activity rapidly decreases to zero
Why does the enzyme change at temperatures too high ?
It vibrates and becomes denatured
When else can an active site denature apart from with temperature?
When the pH is too high
Where could an enzyme that works best in an alkaline pH be released from?
Pancreas into the small intestines
