Required Practical: Chromatography Flashcards

1
Q

Step 1

A

Draw pencil line on chromatography paper 2cm up from bottom

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2
Q

Step 2

A

Mark 5 pencil spots at equal spaces across the line

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3
Q

Step 3

A

Use capillary tube to put a small spot of known food colours and unknown colours onto pencil spots

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4
Q

Step 4

A

Pour 1cm of water into beaker
Attach paper to a rod and lower it in the water so the bottom of it touches the water

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5
Q

Why can the sides of the beaker not touch sides of paper?

A

Intereses with the way water moves

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6
Q

Step 5

A

Put lid on beaker to stop evaporation of water

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7
Q

Step 6

A

Not move the beaker and wait for the water to travel up the paper

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8
Q

Step 7

A

Draw pencil line to mark where water reached on the paper
Hang paper up to dry

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9
Q

Step 8

A

Analyse unknown colour, who’s spots line up with the known colours
Showing what it’s a mixture of

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10
Q

Rf values

A

Ways we identify what the chemicals are in chromatography

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11
Q

Rf calculation

A

Distance the chemical moves
——————————————
Distance the solvent moves

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