Q3: Recombinant DNA Technology

Question 1

The joining of two different DNA molecules from different species resulting to recombined DNA molecule.

Answer 1

recombinant DNA technology

Question 2

Recombinant DNA technology utilizes ____________________ as a cloning vector, a DNA molecule that can carry foreign DNA into a host cell and replicate there.

Answer 2

Bacterial plasmids

Question 3

These are proteins that cut DNA at short, specific sequences called restriction sites.

Answer 3

restriction enzymes

Question 4

It seals the strands of DNA fragments.

Answer 4

DNA ligase

Question 5

This type of restriction enzyme recognizes specific DNA sequences but makes their cut at seemingly random sites that can be as far as 1,000 base pairs away from the recognition site.

Answer 5

Type I

Question 6

This type of restriction enzyme recognizes and cuts directly within the recognition site.

Answer 6

Type II

Question 7

This type of restriction enzyme recognizes specific sequences but make their cut at a different specific location that is usually within about 25 base pairs of the recognition site.

Answer 7

Type III

Question 8

Applications of genetic engineering

Answer 8

• Medical Applications
• Forensic Evidence and Genetic Profiles
• Environmental Cleanup
• Agricultural Applications

Question 9

Medical applications of genetic engineering

Answer 9

• Diagnosis and Treatment of Diseases
• Human Gene Therapy
• Pharmaceutical Products

Question 10

Pharmaceutical Products developed through genetic engineering

Answer 10

• Synthesis of Small Molecules for Use as Drugs
• Protein Production in Cell Cultures
• Protein production by ‘Pharm’ Animals

Question 11

This is used by bacteria to restrict Bacteriophages’ DNA from infecting theirs.

Answer 11

restriction enzymes