Q3: Genetic Engineering Flashcards
Central Dogma of Molecular Biology
- Replication
- Transcription
- Translation
True or False
In bacterial cells, mRNA produced by transcription is immediately translated without additional processing.
True
It is the original RNA transcript which is processed in various ways before leaving the nucleus as mRNA.
pre-mRNA
It is referred to as the direct manipulation of DNA to alter an organism’s characteristics (phenotypes) in a particular way.
genetic engineering
Certain manipulations in genetic engineering
- Altering/Changing one base pair
- Deleting a sequence of DNA
- Introducing an additional copy of a gene (Recombinant DNA)
Genetic Engineering Procedures
- DNA sequencing
- DNA cloning/gene cloning
- gene editing
It exploits the principle of complementary base pairing of DNA to determine the complete nucleotide sequence of a DNA molecule.
DNA sequencing
The peparation of well-defined segments of DNA in multiple identical copies of a specific gene.
DNA cloning/gene cloning
The customizing of a living organism’s genetic sequence by making very specific changes to its DNA using Nucleases.
gene editing
Methods in Genetic Engineering
- Dideoxy Chain Termination Method for Sequencing DNA
- Next-Generation Sequencing
- PCR
- RT-PCR
- CRISPR
What does CRISPR stands for?
Clustered Regularly Interspaced Short Palindromic Repeats
What does RT-PCR stands for?
Reverse Transcriptase - Polymerase Chain Reaction
What are organisms are used as the clone vectors in Recombinant DNA Technology?
bacteria
