Pyruvate

Question 1

Once pyruvate is made in the cytosol where does it go

Answer 1

the mitochondria

Question 2

In the mitochondria, what is the first enzyme pyruvate encounters

Answer 2

pyruvate DH turns it into acetyl-coA makes NADH and CO2

Question 3

Acetyl-coA can be used in

Answer 3

TCA cycle to make more ATP turned into fatty acids

Question 4

Pyruvate DH is inhibited by

Answer 4

acetyl-coA

Question 5

What 5 things does pyruvate DH need to work

Answer 5

thiamine pyrophosphate (thiamine) lipolic aicd conenzyme A (pantothenate) FADH2 (riboflavin) NADH (niacin and some from tryptophan)

Question 6

An alcoholic comes in and you give them IV glucose and they die, why?

Answer 6

lactic acidosis build up of pyruvate no thiamine for pyruvate to move forward goes to lactate instead

Question 7

What 4 pathways can pyruvate go into

Answer 7

1. Made into alanine via alanine aminotransferase (B6), carries amino group to liver from muscle
2. Made into oxaloacetate via pyruvate carboxylase(biotin), replenish TCA or used in gluconeogenesis
3. Made into acetyl-CoA via PDH (B1, B2, B3, B5, lipoic acid) go to TCA
4. Made into lactate via LDH (B3) for anaerobic glycolysis

Question 8

Lactate production is major pathway in

Answer 8

• RBCs
• WBCs
• kidney medulla
• lens
• testes
• cornea

Question 9

What does arsenic inhibit that is important to PDH

Answer 9

Lipoic acid needed as cofactor

• pale skin, skin cancer
• vomiting/diarrhea
• QT prolongation
• garlic breath