Lab techniques

Question 1

lab procedure used to amplify desired DNA fragment

useful in Dx (neonatal HIV, herpes encephalitis)

Answer 1

PCR

Question 2

what does reverse transcriptase PCR do

Answer 2

detects and quantities mRNA levels in a sample

reverse transcriptase creates complementary DNA template that is amplified via standard PCR

Question 3

Southwestern blot identifies

Answer 3

DNA binding proteins using dsDNA probes
c-Jun
c-Fos (leucine zipper motif)

Question 4

technique to assess size, granularity, and protein expression (immunophenotype) of individual cells in a sample

Answer 4

Flow cytometry

Question 5

Commonly used for hematologic abnormalities

• leukemia
• paroxysmal hemoglobinuria
• fetal RBC in mothers blood
• immunodeficiencies (CD4 count in HIV)

Answer 5

Flow cytometry

Question 6

used to profile gene expression levels of thousands of genes simultaneously to study certain diseases and treatments

Answer 6

microarrays

Question 7

immunologic test to detect presence of other specific antibody or antigen in blood sample

Answer 7

ELISA

can have high SN and SP but less SP than Western Blot

Question 8

Can detect microdeletions, translocations, duplications with

Answer 8

FISH