Practical 1: Blood Flashcards
What is the difference between plasma and serum?
Both are liquid components of blood.
Serum is the liquid that remains after the blood has clotted.
Plasma is the liquid that remains when clotting is prevented with the addition of anticoagulants
What is the group that RBC, WBC, and platelets are a part of?
Formed Elements
Which formed element carries O2?
Erythrocytes
Which formed element are part of the immune system?
Leukocytes
Which formed element begins blood clotting?
Platelets
What are most of the cells you see? (Not being pointed to)
Erythrocytes
What’s being pointed to?
Leukocytes
A
Neutrophil
B
Eosinophil
C
Lymphocyte
What are the purple objects?
Lymphocytes
How many erythrocytes in a female?
4.5 million/µL
How many erythrocytes in a male?
5.5 million/µL
What shape are erythrocytes?
Biconcave
What type of nucleus do erythrocytes have?
Anucleated, or enucleated
What is the diameter of an erythrocyte?
7 µm
How long do erythrocytes last/live?
120 days
What do erythrocytes do?
Carry O2 and some CO2
What is the total volume of blood in a female?
5L
What is the volume of blood in a male?
6L
What is hematocrit?
Ratio of erythrocyte volume/total blood volume (including plasma)
How do you measure hematocrit?
- Draw blood into a microtube/vacutainer
- Allow it to clot
- Spin lightly in centrifuge
- Look at the ratio (sink/float)
What is the normal hematocrit level for a female?
42%
What is the normal hematocrit level for a male?
47%
How much hemoglobin do females have?
12-16 g/dL
How much hemoglobin do males have?
13-18 g/dL
How many leukocytes are normally in the blood?
4,800-10,800/µL blood
What is the process of leukocytes becoming mobile and leaving blood to go into the extracellular interstitium and respond to pathogens?
Diapedesis
Where do leukocytes leave through in diapedesis?
The wall of the blood vessel
What is the movement used to diapedese?
Amoeboid movement/motion
What is the difference between granulocytes and agranulocytes?
Granulocytes are leukocytes that contain granules in their cytoplasm
Agranulocytes are leukocytes that lack granules in their cytoplasm
Are neutrophils granulocytes or agranulocytes?
Granulocytes
What’s another name for granulocytes?
Polymorphonuclear leukocytes (Polys)
Are eosinophils granulocytes or agranulocytes?
Granulocytes
Are basophils granulocytes or agranulocytes?
Granulocytes
What’s another name for agranulocytes?
Mononuclear leukocytes
Are lymphocytes granulocytes or agranulocytes?
Agranulocytes
Are monocytes granulocytes or agranulocytes?
Agranulocytes
What percent of leukocytes are neutrophils?
50-70%
What is the normal range of neutrophils in the blood?
2500-8000/µL
How many lobes do the neutrophil nuclei have?
3-7
What color are neutrophil cytoplasm?
Clearish
What are neutrophils role in the immune system?
Phagocytic
What is this?
Neutrophil
What is this?
Neutrophil
What is this?
Neutrophil
What is this?
Neutrophil
What percent of leukocytes are eosinophils?
2-4%
What’s the normal range of eosinophils in the blood?
<500/µL
How many lobes do eosinophil nuclei have?
2
What color is the cytoplasm of eosinophils?
Often pink/red due to granules…About the same color as erythrocytes
What increases the number of eosinophils?
Parasites and allergies
What are eosinophils role in the immune system?
Phagocytic
What is this?
Eosinophil
What is this?
Eosinophil
What is this?
Eosinophil