post lab discussion 5, 6 & 7 Flashcards
which literally means “color writing,” is a method of separating the components of a mixture
chromatography
chromatography is widely used for _______ and _______ analyses or for isolation and recovery of the components of a mixture
qualitative
quantitative
it is the result of chromatography
chromatogram
is the instrument used to conduct chromatography
chromatograph
what are the common types of chromatography
liquid chromatography
gas chromatograph
high performance liquid chromatography
liquid chromatography is also called
column chromatography
identify the type of chromatography:
it is generally used to separate compounds of low volatility
liquid chromatography
identify the type of chromatography:
separates compounds dissolved in a liquid sample and allows qualitative and quantitative analysis of what components and how much of each component are contained in the sample
high performance liquid chromatography
identify the type of chromatography:
term used to describe the group of analytical separation techniques used to analyze volatile substances in the gas phase.
gas chromatography
Chromatography is a method by which a mixture is separated by distributing its components between _______.
2 phases
which phase in the chromatography remains fixed in place
stationary phase
identify the phase in chromatogram:
carries the components of the mixture through the medium being used
mobile phase
identify the phase in chromatogram:
acts as a constraint on many of the components in a mixture, slowing them down to move slower than the mobile phase
stationary phase
in a thin layer chromatography
glass, metal or plastic plates are coated with a _______ serve as the stationary phase
thin layer of adsorbent
paper wick is also known as the
filter paper
filter paper three-quarters of the way around the inside of the developing chamber to help ______ its atmosphere with_______ by wicking solvent into upper region chamber
saturate
solvent vapour
in chromatography,
it is an absorbent of TLC plate
silica gel
in chromatography,
the tiny spots ensures the cleanest separation and does not lead to large tailing spots and poor separation
spotting
it is an equipment used where the compounds usually appear as dark spots
uv lamp
what are the 2 visualization technique for chromatography
uv lamp
iodine visualization
is is a way to visualize colorless
organic compounds that uses their
adsorption of iodine (I2) vapor
iodine visualization
Rf
retardation factor