PMLS LESSON 8 PART 4 Flashcards

1
Q

Refers to Autoclaving or Steam Sterilization

A

wet heat

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2
Q

categories of thermal decontamination

A

wet heat and dry heat

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3
Q

The use of high temperatures to kill microbes

A

thermal decontamination

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4
Q

Most dependable systems available for decontamination of laboratory wastes, sterilization of laboratory glassware, media, and reagents

A

autoclave

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5
Q

What is the autoclaving setting ?

A

Uses saturated steam under 15 psi of pressure and 121 degrees for 30-60 minutes or depending on the material

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6
Q

what are the hazards possessed in Steam sterilization?

A

o Explosive breakage of glass vessels during opening and
unloading
o Steam burns
o Burning results from physical contact
o Metal containers, glass containers, etc.
o Aerosolization of hazardous fumes

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7
Q

What should containers should be used during steam sterilization and what should be the conditions?

A

Polypropylene bag loosely closed and glassware containing materials should be looselycapped or loosely covered in foil

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8
Q

sterilize items that may be damaged by moist heat or that are impenetrable to moist heat

A

dry heat

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9
Q

Relies on conduction to heat materials for periods of 2 hours at 160 degrees Celsius or 1 hour at 170 degrees Celsius

A

dry oven

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10
Q

when is dry heat used or a dry oven used?

A

employed when decontaminating materials that may be ruined by moist/wet heat

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11
Q

setup of dry oven or temp of dry oven?

A

160 degrees celsius for 2 hours and 170 degrees for 1 hour

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12
Q

Treatment of choice for animal bedding, carcasses, and
pathological wastes; but not plastics

A

incineration

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13
Q

ionizing Radiation will destroy microorganisms but is not a practical tool for laboratory use

A

radiation

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14
Q

Considered a controversial practice due to environment implications

A

incineration

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15
Q

will destroy microorganisms but is not a practical tool for laboratory use

A

radiation

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16
Q

what is replaced for ionizing radiation?

A

non-ionizing radiation, UVC Band of UV rRadiation

17
Q

reduce levels of airborne microorganisms and maintain good air hygiene in air locks

A

UV-C band of UV radiation

18
Q

is not used because it might expose the laboratory worker to dangerous levels of radiation

A

ionizing radiation