Path 5 Flashcards
How many children are HIV +ve? (2012) How many of them are in Sub-Saharan Africa?
Total 3.3 Million
2.9 Million in SSA
What % of people living wit HIV are children? (2010)
1 in 10
How do children get HIV?
> 90% due to mother-child transmission
but also:
-child sexual abuse
- exchanging sex for food/shelter
What % of deaths under 5 in S-Africa are due to HIV?
~35%
Sx of HIV infection in children
- failure to thrive
- (chronic bilateral) parotid enlargement
- (drastic) lymphadenopathy
- rashes (molloscum, scabies (can even be not itchy b/c immune response is needed for itchiness)/ nappy rash
- herpes zoster infection
- hepato-/splenomegaly
- (severe) oral thrush
- TB/severe pneumonia/LIP/Pneumocystitis carinii
- recurrent/persistent diarrhoea
- clubbing
- CNS involvement (
LIP
lymphoid interstitial pneumonitis
(cannot be distinguished from TB on CXR)
CNS changes in children infected with HIV
Basal ganglia calcification
White matter changes
Atrophy
Vasculopathy / Strokes
How can HIV be transmitted from mother to child?
in utero
intra partum
breastfeeding
HIV - what is MTCT?
mother to child transmission
What is a major risk factor for HIV MTCT?
Maternal plasma viral load
What is the pattern of viraemia (with viral load numbers) in HIV infection in humans?
- rapid rise and initial peak at 10^6 copies/ml
- drop and set point at 10^3 - 10^5 copies/ml
- rise of copies/ml in late disease
Stats about risk of getting HIV via breast feeding?
- 4% transmission risk for every 6 months of breast-feeding
- risk of of HIV transmission must be balanced against risk of increased mortality from formula feeding
- Risk from drinking 1 litre of breast milk = risk from one episode of unprotected sex
- If IMR is > 40 / 1,000 live births, recommend exclusive breast feeding + ARVs for mother or baby [WHO 2010]
(this is about sub Saharan Africa )
What is IMR?
infant mortality rate
Approaches to prevent HIV infection in infants
- prevention of HIV in parents
- prevention of unintended pregnancies in HIV +ve women
- prevention of MTCT
- Care and support for HIV-infected women, their infants and their families
What classes of antiretrovirals are currently used in children with HIV in Africa?
- non-nucleoside reverse transcriptase inhibitors
- nucleoside analogues
- nucleotide analogues
- protease inhibitors
What medication group ends with -gravir?
integrase inhibitors
Name examples of integrase inhibitors
Raltegravir
Elvitegravir
Dolutegravir
When is Fontana Stain positive?
melanoma
What does a positive fontana strain indicate?
melanoma
When is Congo red stain positive? What birefringence is seen?
amyloidosis
+ apple green birefringence
What stain in +ve in amyloidosis?
Congo red + apple green birefringence
What stain is positive in Wilson’s disease?
Rhodanine (golden brown against blue counterstain)
+ve for copper
When is Rhodanine stain +ve? What colour does it change to?
Wilson’s disease
Golden brown against blue counterstain
(+ve for copper)
Prussian blue stain +ve - diagnosis?
Haemochromatosis
(+ve for iron)
Which stains are positive in haemochromatosis?
Prussian blue and Perl’s stain
(both are +ve for iron)
Perl’s stain +ve - diagnosis? What makes it +ve?
haemochromatosis
iron
Cytokeratin stain +ve - what is it?
positive for epithelial cells -> carcinoma
CD45 stain +ve - what is it?
+ve for lymphoid cells / lymphocytes
Ziehl Neelsen Stain +ve - diagnosis?
What colour change can be seen?
+ve for acid fast bacilli - TB
red against a blue background
Rhodamine-Auramine stain +ve - diagnosis and colour
TB
colour: bright yellow
What stains are +ve in TB
Ziehl Neelsen and Rhodamine-Auramine
What stain is positive in pneumocystitis jirovecii? What can be seen?
Gomori’s methanamine silver stain
flying saucer shaped cysts
Gomori’s methanamine silver stain with flying saucer shaped cysts - diganosis?
Pneumocystis jirovecii
What type of pathogen is Pneumocystis jirovecii?
fungus
What stain to test for cryptosporidium parvum?
Modified Kinyoung acid fast stain
Modified Kinyoung acid fast stain - diagnosis?
Cryptosporidium parvum
When is India ink stain +ve ? What can be seen?
Cryptococcus neoformans
(yeast cells surrounded by halos)
What stain is +ve in Cryptococcus neoformans and what can be seen?
India ink stain
(yeast cells surrounded by halos)
when is Giemsa stain +ve?
Chlamydia psittaci (cytoplasmic inclusions seen)
Which stain is +ve in Chlamydia psittaci and what can be seen?
Giemsa stain
(cytoplasmic inclusions seen)
Fite stain +ve - diagnosis?
Mycobacterium leprae
What stain is +ve in mycobacterium leprae?
What are the symptoms of Zika virus?
1 in 5 infected people have symptoms
- fever
- rash
- joint pain
- headache
- conjunctivitis
- muscle pain
Sx last for several days to a week
How can you get Zika virus?
- mosquito bite
- mother to child transmission in pregnancy
- sex
- blood transfusion
HOW does Zika virus cause damage in pregnancy?
- crosses the placenta
- targets neuronal progenitor cells (neuronal growth, proliferation, migration is disrupted)
What problems does Zika virus cause to the baby in pregnancy?
- microcephaly
- craniofacial disproportion
- skull abnormalities
- damage to the brain
- seizures
- problems with hearing (?SN hearing loss)
- problems with vision
- problems with feeding (issues swallowing)
- problems with moving limbs and body
Advice for women regarding Zika and pregnancy
- All travellers – bite avoidance
- Pregnant women – avoid travel to areas with current transmission
- Avoid conception for 2 – 6 months after travel (prolonged viral shedding in semen)
- Testing only if symptomatic or abnormalities identified on antenatal USS
Is a flare-up of genital herpes in pregnancy dangerous?
No
you have antibodies that protect you and the baby
only primary infections are worrying
What are the risks of primary HSV infection in pregnancy to the baby?
- miscarriage
- congenital abnormalities (ventriculomegaly, CNS abnormalities)
- preterm birth
What cancer does EBV infection predispose you to?
Burkitt’s lymphoma
What kind of virus is CMV?
enveloped
dsDNA genome
In which cells does CMV remain latent?
Lies latent in monocytes
and dendritic cells
which cells does EBV remain latent in?
B-cells
epithelial cells
NK cells
T-cells