Part K: Lecture 45

Question 1

FISH stands for ___

Answer 1

fluorescence in situ hybridization

Question 2

FISH purpose

Answer 2

identify genes or chromosomes amongst interphase or metaphase chromosomes

Question 3

FISH can use cells from ____

Answer 3

adult, child, embryo

Question 4

DAPI is ___

Answer 4

a synthetic molecule that has a high affinity for DNA and fluoresces blue

Question 5

hybridization probes are made from ___

Answer 5

BAC clones (partially sequenced)

Question 6

random prime labelling reaction =

Answer 6

BAC (known dsDNA) + labeled nt = hybridization probe (ssDNA)

Question 7

hybridization probe recognizes ____

Answer 7

one sequence region of DNA (how many exist)

Question 8

hybridization probe is small but BAC can help us recognize region of DNA that are ____long

Answer 8

100 000 bp

Question 9

FISH with centromere probes purpose

Answer 9

count the chromosomes (check for aneuploidy)
-1 colour probe

Question 10

FISH with gene probes purpose

Answer 10

determine location and presence of gene or region (check for deletion, insertion etc.)
-1 colour probe

Question 11

FISH with chromosome paint probes purpose

Answer 11

identify chromosome rearrangements (check for translocation)
-1 colour probe

Question 12

pericentric inversion would need probes of ____

Answer 12

two different colours

Question 13

multicolour FISH purpose

Answer 13

identify all chromosomes

Question 14

how many different chromosomes do humans have ?

Answer 14

24 (22 pairs of autosomes and X,Y)

Question 15

multicolor FISH probes have ___

Answer 15

multicolours (1,2,3 colours)