Part D: Lecture 30 Flashcards
classical genetics began with ____ in 1865
Mendel
Classical genetics is the science of ____
solving biological questions by examining the offspring of living organisms (in vivo)
In vivo experiments are done using ____
living organisms (“in life”)
Molecular genetics is the science of _____
solving biological questions using DNA, RNA, and proteins isolated from organisms (in vitro)
Molecular genetics began in ____ with _____
1970 with the discovery of restriction enzymes
In vitro experiments are done ____
in a glass test tube
Falcon tubes (made for ___)
originally made by falcon for centrifuges
Epp tubes (made for ___)
originally made by Eppendorf for microcentrifuges
Type of nucleic acids used for nucleic acid isolation (5)
genomic DNA
plasmid DNA (prok. only)
organelle DNA (euk. only)
total RNA
mRNA
Methods used for nucleic acid isolation (2)
DIY (cheaper)
Kit (faster)
DIY isolation of nucleic acid
uses reagents present in lab (ex. NaOH)
Genomic DNA isolation kit uses _____
blood samples
Genomic DNA isolation Kit method
- Release DNA (Enzymatic digestion)
- Bind DNA (Add ethanol and spin)
- Wash column (Wash and spin twice)
- Elute DNA (Spin 1 minute)
Which is easier to isolate? RNA or DNA? Why?
DNA (more stable)
Harder to isolate RNA because it is unstable temporary molecule)
After nucleic acid isolation, DNA is treated with ____
enzymes from Biotech companies
