Nucleic Acid Word Stimulants Flashcards
DNA
Double Helix Replicated by parent DNA Thousands of bases Carries genetic info from parents Phosphodiester bond
DNA pairs
Adenine - Thymine
Guanine - Cytosine
1:1 ratio
Thousands of bases
Phosphodiester Bond
Condensation reactions
Phosphate C3 - Sugar C5 of another
DNA location
Mainly Nucleus; some Mitochondria / chloroplast
Nucleotide Structure
Pentose Sugar (deoxyribose/ribose)
Phosphate group
Nitrogen-containing organic group
Complementary Base Pairs
Adenine - Thymine/Uracil
Guanine - Cytosine
Hydrogen bonds (3 GC) (2 AT/U)
RNA
Single Sranded (phsophodiester strand) Transcribed against DNA template
RNA Pairs
Adenine - Uracil
Guanine - Cytosine
No base ratio
RNA Location
Made in nucleus
Found in cytoplasm
tRNA Structure
Transfer RNA
70-90 bases
Clover shaped strand
tRNA Role
Binds to amino acids
Carriers to mRNA/ribose complex
mRNA Structure
Few hundred>thousands of bases
mRNA Role
Genetic material
Nucleus>Ribosome (polypeptide synthesised)
DNA replication location
Nucleus
S phase of Interphase
DNA Replication Steps
DNA double strand ‘unzips’ - h bonds break
DNA helicase
Free nucleotides in nucleus joins to complementary bases on tRNA
DNA polymerase (condensation reaction)
Two new separate strands
DNA Helicase
‘Unzips’ DNA double strand
Breaks hydrogen bonds
Hydrolysis Reactions
DNA polymerase
Joins complementary nucleotides
Condensation Reaction
Semi-Conservative Model
Old strand (parent - genetic continuity) Two new strands (1 new strand, 1 old)
Watson-Crick Model
Isotopes
N14 - light, top of tube
N15 - heavy, bottom of tube
Centrifuged 1st (mixed) & 2nd generation (1 light, 1 mixed)
ATP
Adenosine Triophosphate
ADP
Adenine Diphophate
Inorganic Phophate
Pi
Phosphorylates other compounds - more reactive
Energy
Some heat (ATP>ADP) Light dependent photosynthesis, sugars oxidised by cellular respiration (ADP>ATP)
ATP Structure
Adenine
Ribose
3 Phsophate groups