Nucleic Acid (DNA Isolation) Flashcards
carries the genetic
instructions essential for the
growth, development, functioning,
and reproduction of all living
organisms
deoxyribonucleic acid
DNA is made up of
nucleotides
DNA nucleotides has three parts
2-deoxyribose
phosphate group
nitrogenous bases
DNA isolation is used for (7)
polymerase chain reaction (PCR)
DNA sequencing
gene cloning
genetic engineering
diagnosis of genetic disorders
production of vaccines
advancements in personalized medicine
why is onions used for the experiment
(1) large number of cells
(2) relatively low starch content
(3) easy to obtain
why is strawberries also used
soft and easy to pulverize
large genomes; octoploid
easy to obtain
having eight sets of chromosomes, or eight times the basic haploid chromosome number
octoploid
how many chromosomes have strawberries have in total
56 chromosomes
each cell in a strawberry contains ___ sets of chromosomes
eight
has been successfully
extracted in microgravity aboard the
International Space Station
DNA extraction
DNA extraction has been successfully
extracted in ____ aboard the
International Space Station
microgravity
DNA extraction has been successfully
extracted in microgravity aboard the
International Space Station
materials used for the experiment
50 g of Onion
Blender
Buffer solution
95% cool ethanol
buffer solution is made up of
detergent
NaCl
50 ml distilled water
equipment used for the experiment
glass rod
spatula
petri dish
hot plate
graduated cylinders
beakers
measure ___ of onion
50g
size of onion
diced (not too fine)
can also be done to cut the onion
blend
what happens to the onion if exposed to buffer solution
cell lysis
buffer solution amounts
liquid detergent
2-3 tbsp
buffer solution amounts
sodium chloride
4-5 spatula
buffer solution amounts
water
50 ml distilled water
Add the buffer solution to the onion pulp then ___
stir
Heat the mixture to ____°C for 5–10 minutes using a hot plate or water bath
40-50oC