Molecular Techniques Flashcards
1
Q
- PCR requires:
a. Product annealing
b. Primer denaturation
c. Polymerase denaturation
d. Primer annealing
A
b. Primer denaturation ?
2
Q
- Restriction fragment length polymorphisms:
a. Restrict the length of polymorphisms
b. Require treatment with a restriction enzyme for
visualization
c. Are polymorphisms in restriction enzymes
d. Are commonly visualized by massively parallel
sequencing
A
b. Require treatment with a restriction enzyme for
visualization ?
3
Q
- Which method does not use electrophoresis?
a. Single nucleotide extension
b. PCR/RFLP
c. Dideoxy-termination sequencing
d. Pyrosequencing
A
d. Pyrosequencing
4
Q
- Hybridization probes:
a. Are the same as hydrolysis probes
b. Can consist of one or two probes
c. Irreversibly lose fluorescence once hybridized
d. Never function by resonance energy transfer
A
5
Q
- Real time PCR:
a. Is a qualitative assay
b. Can be observed during amplification
c. Never uses fluorescence
d. Requires melting analysis
A
6
Q
- How fast can PCR be performed?
a. In about an hour
b. In 15 minutes
c. In 5 minutes
d. In less than 1 minute
A
7
Q
- Massively parallel sequencing:
a. Costs more per base than dideoxy sequencing
b. Is simpler than dideoxy sequencing
c. Is more descriptive than next generation sequencing
d. Has a fast turnaround time
A
8
Q
- Single molecule sequencing:
a. Can produce longer read lengths than typical massively parallel sequencing
b. Is less informative than massively parallel sequencing
c. Makes it difficult to haplotype 2 variants
d. Is impossible
A
9
Q
- DNA melting analysis:
a. Is expensive and time consuming
b. Is performed before PCR
c. Plots fluorescence and temperature to produce melting curves
d. Is destructive
A
10
Q
- Hydrolysis probes:
a. Are reversible
b. Increase fluorescence after the probe is hydrolyzed
c. Are very good for melting analysis
d. Are less specific than DNA dyes
A
