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Miscellaneous Gram Negative Bacteria Flashcards

1
Q

Disease caused by Pajaroellerobacter abortibovis

A

Foothill abortion in cattle aka epizootic bovine abortion

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2
Q

Where is Pajaroellerbacter abortibovis endemic?

A

California, Nevada and Oregon

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3
Q

How is Pajaroellerobacter abortibovis transmitted?

A

soft tick

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4
Q

How long does immunity against Pajaroellerbacter abortibovis last?

A

1-3 years

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5
Q

Incubation period for Parajorellerobacter abortibovis

A

lasts more than 100 days

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6
Q

When is the fetus most at risk due to Parajorellerobacter abortibovis

A

45 days prior to breeding through 5-6 months of gestation

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7
Q

General pathogenesis of Pajaroellerobacter abortibovis

A

aborted fetus in excellent condition
extensive ascities with excessive fibrin
skin lesions
petechial hemorrhages on mucosal memnranes and thymus

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8
Q

How is Pajaroellerobacter abortibovis diagnosed?

A

tentative diagnosis based on unique fetal pathology
PCR targeting 16S bacterial ribosomal gene

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9
Q

Can Pajaroellerobacter abortibovis be cultured?

A

NO

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10
Q

Staining methods use to visualize Pajaroellerobacter abortibovis

A

gram neg but not well
modified steiner silver stain
immunofluorescence

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11
Q

Is there a vaccine for Pajaroellerobacter abortibovis?

A

yes - live vaccine

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12
Q

Causative agent of red-leg disease in frogs

A

Aeromonas hydrophilia

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12
Q

Causative agent of necrotic stomatitis in snakes and septicemia

A

Aeromonas hydrophilia

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13
Q

Causative agent of skin lesions and hemorrhagic septicemia in eels, cryprids and pike

A

Aeromonas hydrophilia

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14
Q

Causative agent of swin bladder inflammation in pike and grass carp

A

Aeromonas hydrophilia

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15
Q

Causative agent of neonatal septicemia in dogs

A

Aeromonas hydrophilia

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16
Q

Causative agent of mastitis in cattle (misc gram neg)

A

Aeromonas hydrophilia

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17
Q

Causative agent of septicemia in turkeys (misc gram neg)

A

Aeromonas hydrophilia

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18
Q

Causative agent of diarrhea in pigs (misc gram neg)

A

Aeromonas hydrophilia

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19
Q

Causative agent of furunculosis in salmonids

A

Aeromonas salmonicida subsp. salmonicida

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20
Q

Causative agent of ulcer disease in carp

A

Aeromonas salmonicida subsp. salmonicida

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21
Q

What is the habitat of Aeromonas spp?

A

freshwater, sweage, soil

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22
Q

Is aeromonas hydophilia part of the normal flora of freshwater fish?

A

yes
opportunity pathogen

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23
Q

Is aeromonas salmonicida an obligate parasite?

A

yes of salmoid fish

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24
Q

Virulence factors of aeromonas hydrophilia

A

adhesins
exoenzymes
hemolysins
enterotoxins
acetyltransferase system

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25
Q

Virulence factors of aeromonas salmonicida

A

outer protein coat
adhesin
type I and IV pili
type III secretory system

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26
Q

Aeromonas spp direct microscopy

A

gram neg straight rods
fluorescent antibody test for salmonicida

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27
Q

Are aeromonas spp anerobic or aerobic?

A

aerobic

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28
Q

Culture media used for aeromonas hydrophilia

A

Blood agar
MAC
blood agar + 10mg/L ampicillin
CIN

29
Q

Culure media used for aeromonas salmonicida

A

blood agar and furunculosis agar at 25C for 48hrs

30
Q

Aeromonas hydrophilia colony morphology

A

large, flat, grey colonies
betahemolysis
punget foul odor
MAC - pale colonies

31
Q

How are aeromonas spp, plesiomonas spp, and vibrio spp differentiated?

A

biochemical testing

32
Q

What miscellaneous gram negative bacteria causes opportunistic infections in fish?

A

Plesiomonas shigelloides

33
Q

Virulence factors of Plesiomonas shigelloides

A

heat-stable and heat-liable enterotoxins

34
Q

Culture medium used for Plesiomonas shigelloides

A

NA
BA
MAC - nonlactose fermenting

35
Q

Hemolysis of Plesiomonas shigelloides

A

non-hemolytic

36
Q

Antibiotic resistance of Plesiomonas shigelloides

A

resistant to beta lactams

37
Q

What disease does vibrio metschnikovii cause in chickens/birds?

A

enertic disease

38
Q

What disease does vibrio anguillarum cause in salt-water eels and fish?

A

skin necrosis
high mortality

39
Q

Mortlity rate of marine vibrios in fish

A

up to 100%

40
Q

Culture media used for Vibrio anguillarum

A

BA + NA base 2.0% NaCl at 20C for 48hrs

41
Q

Culture media used for Vibrio parahaemolyticus

42
Q

Culture media used for vibrio chloerae and other enteric vibrios

A

grow on mannitol salt agar 7.5% NaCl

43
Q

Colony morphology of vibrio metschnikovii

A

smooth, transparent colonies
hemolytic
grows poorly on MAC

44
Q

Colony morphology of vibrio anguilarum

A

smooth colonies
hemolytic

45
Q

Colony morphology of vibrio parahemolyticus

A

non-hemolytic
green colonies on TCBS
ferments mannitol

46
Q

Reservoirs of Streptobacillus moniliformis

A

rats and small carnivores

47
Q

Disease caused by Streptobacillus moniliformis

A

Rate-bite Fever aka Haverhill fever

48
Q

Staining methods used for visualizing Streptobacillus moniliformis

A

does not stain well with Gram Stain
dilute carbol fuschin
giemsa stain
immunofluorescence

49
Q

Culture media used for Steprobacillus moniliformis

A

brain heart infusion base enriched with 15% blood, 20% horse or calf serum or 5% ascitic fluid

50
Q

Colony morphology of Streptobacillus moniliformis

A

non-hemolytic
round
grey
smooth glistening

51
Q

Microscopic appearance of Streptobacillus moniliformis

A

pleomorphic gram neg rod
filamentous with knobby irregularities
non-motile

52
Q

Biochemical reactivity of Streptobacillus moniliformis

A

unreactive biochemically

53
Q

Disease caused by ornithobacterium rhinotracheale

A

respiratory disease in poultry - chickens and turkeys

54
Q

Co-infections commonly found in ornithobacterium rhinotracheale

A

E. coli
Bordetella avium

55
Q

Culturing requirements for ornithobacterium rhinotracheale

A

5-10% BA supplemented with 5-10% CO2
does not grow on MAC

56
Q

Disease caused by Riemerella anatipestifer

A

New duck disease aka duck septicemia - ducks and geese

57
Q

Mortality rate of Riemerella anatipestifer

58
Q

Characteristics of Riemerella anatipestifer

A

gram-neg rod: singly, in pairs, occasionally in chains
non-motile
non-spore forming
capsule

59
Q

How to demonstrate Riemerella anatipestifer capsule

A

india ink stain

60
Q

Transmission of Riemerella anatipestifer

A

oral or nasal route
skin wounds particularly on feet

61
Q

Virulence factors of Riemerella anatipestifer

A

VampD
OmpA
CAMP cohemolysin
Nicotinamidase PncA
LPS
biofilm formation

62
Q

Clinical signs of Riemerella anatipestifers

A

listlessness
ocular and nasal discharge
sneezing
greenish diarrhea
ataxia
torticollis
tremors of head and neck
coma

63
Q

Pathology of Riemerella anatipestifer

A

fibrinous exudate in serosal cavities
mucopurulent exudate in nasal sinuses
caseous exudate in oviducts
localized skin lesions

64
Q

Culture media for Riemerella anatipestifer

A

BA in 5-10% CO2
No growth on MAC

65
Q

Colony morphology of Riemerella anatipestifer

A

small, transparent, glistening colonies
non-hemolytic

66
Q

How to serologically classify Riemerella anatipestifer

A

gel precipitation test or agglutination

67
Q

Differential diagnosis of Riemerella anatipestifer

A

Pasteruella multocida
Escherichia coli
Enterococcus faecalis
Salmonella spp.

68
Q

Treatment for Riemerella anatipestifer infection

A

sulfonamides in feed or drinking water
enrofloxacin
ceftiofur

69
Q

Control and prevention of Riemerella anatipestifer

A

inactivated vaccines
live avirulent vaccine
management and sanitation

Bacteriology (26 decks)

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