Brucella Flashcards

1
Q

Brucella general characteristics

A

gram neg coccobacilli
aerobic, non-fermentative
facultative intracellular
capnophillic

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2
Q

Is Brucella zoonotic?

A

YES

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3
Q

Does brucella require enriched media?

A

yes - media must be enriched with blood or serum

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4
Q

How is Brucella transmitted?

A

oral or venereal

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5
Q

Clinical Signs of Brucellosis

A

abortion + retained placenta
infertility
mastitis
orchitis
epididymitis
seminal abnormalities
testicular atrophy
affects joints

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6
Q

Lesions associated with Brucellosis

A

placentitis
epididymitis
endometritis
fetus: endema and congestion of lungs

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7
Q

Why is diagnosis of brucellosis difficult in bulls and non-pregnant females?

A

typically they are asymptomatic

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8
Q

Samples used for cultural examination for brucellosis

A

fetal stomach contents
placenta
vaginal discharge
semen
milk
lymph nodes

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9
Q

What type of staining is used when diagnosing brucellosis?

A

modified Ziehl-Neelsen stain
positive smears: small, red staining coccobacilli in clumps

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10
Q

What diagnostic test is used to conifrm diagnosis of brucellosis?

A

PCR

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11
Q

Serological tests used to diagnose brucellosis

A

serum agglutination test: detects IgM and IgG2
rose-bengal plate test
milk ring test: neg = blue
complement fixation test

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12
Q

Which strain is used more commonly for vaccination against Brucella abortus?

A

strain 19
live attenuated
vaccinate female calves 4-12 mo

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12
Q

Why are male calves not vaccinated with Strain 19 B. abortus vaccine?

A

causes infertility

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13
Q

What are the concerns with the RB51 strain used to vaccinate against B. abortus?

A

can infect humans via accidental exposure
rough mutant
used in adults

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14
Q

Clinical signs of brucellosis in swine (caused by B. suis)

A

abortion
sterility
still births
spondylitis
abscesses

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15
Q

What is unique about brucella ovis?

A

least pathogenic

16
Q

Clinial signs of brucellosis in sheep

A

unilateral epipdidymitis
abortion
infertility

17
Q

Which species does brucella canis infect?

A

only dogs
highly contagious

18
Q

Clinical signs of brucellosis in male dogs

A

epididymitis
prostatitis
chronic: testicular atrophy and sterility

19
Q

Serological diagnosis of canine brucellosis

A

positive agglutination test

20
Q

Treatment for canine brucellosis

A

antibiotics: tetracyclines and getamicin
for 3 months
difficult to cure - may relapse

21
Q

Is treatment receommended for breeding dogs positive for brucellosis?

A

No recommend humane euthanasia

22
Q

Is there a vaccine available for canine brucellosis?

A

NO

23
Q

What is the causative agent of Brucellosis in horses?

A

B. abortus

24
Q

What are the clinical signs associated with brucellosis in horses?

A

abortion is rare
artitis is common
associated with poll evil

25
Q

What is the causative agent of brucellosis in goats and sheep?

A

Brucella melitensis

26
Q

Clinical signs associated with bricellosis in goats and sheep

A

abortion
orchitis