(M) Week 9: Eosinophil Count Flashcards
Causes of Eosinopenia
- Cushing’s Disease (Hyperadrenalism)
- Shock
- Administration of ACTH
Causes of Eosinophilia
Allergic reaction
Parasitic infestations
Brucellosis
Certain leukemia
TOF. Eosinophil COUNT is still used up to this day.
F (obselete)
What type of method is the absolute counth method
Indirect
Formula for the absolute count method
Differential count of Eo % x WBC ct
Normal Reference Value for Eo % in WBC diff count
0-7%
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Principle: Dilute the blood at specified dilution and count the EOSINOPHIL using a counting chamber
Direct Method
Reagents available for Direct Method
Phyloxine diluting fluid
Pilot’s solution Randolph’s stain
Objective: To stain the granules of eosinophil
Components: Phyloxine, Water, Sodium
Carbonate, and Propylene glycol
Phyloxine diluting fluid
Phyloxine diluting fluid
Components:
* Stain the granules of Eo
* Lyse WBC except the Eo
* Lyse the RBC
- Phyloxine (red)
- Water and sodium carbonate
- Propylene glycol
Anticoagulant for Pilot’s solution
Heparin
il
Same with Phyloxine diluting fluid but with
methylene blue and phyloxine.
Randolph’s stain
What pipette is used for Eo
WBC pipette or unopette pipette system
2 TYPES of Hemocytometer
- Fuchs-rosenthal
- Speirs-Levey
Depth of Speirs-Levy and Chamber is both is?
0.2mm
