(M) Week 7: CBC and other procedures I part 2 Flashcards
familiarize the reagents and equipment for blood smear
- slides
- hematological stains
- microscope
- schilling counter
what are the stains used in differential counts
- Wright’s
- Giemsa
- Leishman’s
- other romanowsky stain
Romanowsky stain’s dip method uses which reagents
a. methanol (fixative for blood smear)
b. eosin (primary stain)
c. methylene blue (secondary stain)
d. distilled water (washes out excess stain)
rack method
stains that are too acidic changes the __________ or the ____________ of the cell
appearance or morphology of the cell
rack method
stains that are too acidic changes the cell color to
bright red or pink
rack method
stains that are too basic or alkaline can appear (darker / lighter in color)
darker
rack method
what helps maintain the integrity of the blood smear
washing
what type of microscope is used for differential count
bright-field
what type of Romanowsky stain is routinely used
Wright’s
which type of Romanowsky stain is used in searching for malarial parasites
Giemsa
What causes falsely increased WBC when the patient is crying?
WBC in the marginating pool migrates to the circulating pool
familiarize the procedure
- check slide identification
- perform patient specimen orientation
- perform low power scan to review blood film adequacy
- perform diff leukocyte count
- classify 100 leukocytes using the battlement track method
- report results of the 100 cells classified as percentage
- keep separate count of nucleated RBCs
- Note and report the abnormal leukocyte morphology
- Identify and report any miscellaneous non-leukocyte abnormal cells such as endothelial cells, basket cells, or NRBCs
in what areas of the smear do we perform cell counting?
uniform monolayer of smear
What are the criteria for leukocyte identification
- cell size
- nuclear - cytoplasmic ratio
- cytoplasmic characteristics
- nuclear characteristics
what does high NC ratio mean in leukocyte identification
immature cell
(nucleus occupies most cell areas with only a small rim)
what does low NC ratio mean in leukocyte identification
mature cell
nucleus is small in relation to volume of cytoplasm
what are the three cytoplasmic characteristics
a. color of background cytoplasm
b. presence of absence of granules
c. color and size of granules
what are the nuclear characteristics
- shape
b. color
c. chromatin pattern
d. presence or absence of nucleus
neutrophil with more than 5 lobules
hypersegmented
neutrophil with less than 3 lobules
hyposegmented
sign of maturation in neutrophil
conspicuous filament / constrictioni that connects segments
an immature neutrophil (no restriction)
- c or s shaped
band neutrophil
there is a slight indentation, kidney bean-shaped with lilac granules
metamyelocyte
has fine chromatin sturcture and uniform nucleus, cytoplasm appears sky blue in color
lymphocytes