F(4) CD markers Flashcards

1
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD 34

A

a

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1
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD 117

A

a

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2
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

Terminal Deoxynucleotidyl transferase

A

a

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3
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD 33

A

B

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4
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD 13

A

B

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5
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD 15

A

B

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6
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD14

A

B

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7
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD71

A

C

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8
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

Glycophorin A

A

C

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9
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD41

A

D

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10
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD42

A

D

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11
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD61

A

D

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12
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD19

A

e

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13
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD 20

A

B

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14
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD22

A

E

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15
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

K light chain

16
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

Lambda light chin

17
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD2

18
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD3

19
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD4

20
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD5

21
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD7

22
Q

match the marker to the lineage

a. immature
b. granulocyte / monocyte
c. erythroid
d. megakaryocyte
e. B lymphocyte
f. T lymphocyte

CD8

23
Q

What are the CD markers under the immature lineage

A

CD34
CD117
Terminal deoxynuleotidyl transferase

24
What are the CD markers under the granulocyte / monocyte lineage
CD 33 CD 13 CD 15 CD 14
25
What are the CD markers under the Erythroid lineage
CD 71 Glycophorin A
26
What are the CD markers under the Megakaryocye lineage
CD 41 CD 42 CD 61
27
What are the CD markers under the B lymphocyte lineage
CD 19 CD 20 CD 22 K light chain Lambda light chain
28
What are the CD markers under the T lymphocyte lineage
CD 2 CD 3 CD 4 CD 5 CD 7 CD 8
29
# CELL POPULATION IDENTIFIED BY FLOW CYTOMETRY Negative for CD45 CD71 transferrin receptor (low in reticulocyte) Glycophorin A
Erythroid lineage
30
# CELL POPULATION IDENTIFIED BY FLOW CYTOMETRY CD41 and CD61 (gpIIb / IIIa) - first marker of megakaryocytes differentiation CD34 31,36 present in Megakaryoblast CD42, 62p, and 36 present in platelets and Megakaryocyte
Megakaryocytic lineage
31
* CD34, terminal deoxynucleotidyl transferase (Tdt) present both in T cell and B cell * CD19, cytoplasmic CD22 and * CD79 earliest B cell marker * CD10 is acquired as B cell * precursor matures but declines as B cell reaches full maturation * U chain immature B cell marker * CD 20 marker of mature B cell * Kappa and lambda chain marker of mature B cell * CD34, terminal deoxynucleotidyl transferase (Tdt) present both in T cell and B cell * CD2, 7, cytoplasmic CD 3 marker of immature T cell * CD2 ,7 and 56 is present in NK cell * As T cell matures CD1a and CD 5 and co expression of CD4 and 8 appeared * Finally CD 3 appears with subsequent loss of CD 4 and 8
Lymphoid lineage