Lecture 9 Microbial Responses Flashcards

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1
Q

Ideal growth conditions for a microbe

A

A temperature where all their enzymes are folded properly and working at the optimum rate

Plenty of food

The correct atmosphere for their own type of respiration

Available water

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2
Q

Environmental condition that dictate microbial growth and distribution and habitats of microbes

A

Temperature
pH
Osmolarity (water concentration/availability)
Oxygen

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3
Q

What does temperature control

A

Chemical and enzymatic reaction

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4
Q

What happens to enzymes and protein when above the maximum temperature

A

Denatured

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5
Q

What happens to the cell membrane when below the minimum temperature

A

May no longer function- required for nutrient transport and energy production

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6
Q

Psychrophiles have an optimal growth temperature of

A

15 degrees celcius or lower
Found in environments that are constantly cold and rapidly due at room temp

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7
Q

Freezing prevents microbial growth but doesn’t always cause what

A

Cell death

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8
Q

Effects of freezing

A

•Dehydration
•Ice crystal formation

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9
Q

Above 65 degrees celcius only what exists

A

prokaryotic life (bacteria and archaea)

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10
Q

Optimum growth temperature of Hyperthermophiles

A

> 80 degrees celcius

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11
Q

What is more thermophilic bacteria or archaea

A

Archaea

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12
Q

What happens to double stranded DNA at high temperature

A

They seperate

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13
Q

Name the enzymes in Hyperthermophiles that prevents double stranded DNA molecules from separating at high temperature

A

Reverse DNA Gyrase

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14
Q

Reverse DNA Gyrase is absent in organism that grow below what temperature

A

80 degrees

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15
Q

What does pH refer to

A

concentration of hydrogen ions (H+) in a solution and is commonly expressed in terms of the pH scale, which is a log scale.

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16
Q

Low pH corresponds to

A

high hydrogen ion concentration

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17
Q

High pH corresponds to

A

low hydrogen ion concentration.

18
Q

What is the formula for calculating pH

A

pH = -log [H+]

19
Q

Most microorganisms grow best at what pH

A

6-8(Neutrophiles)

20
Q

Acidophiles are ___ loving

A

Acid loving

21
Q

Alkali phones are _____ loving

A

Alkaline loving

22
Q

Internal cell pH must be close to what type of pH

A

Neural between a pH of 5-9

23
Q

What happens to cell macromolecules(enzymes , proteins and nucleic acid) at pH extreme

A

They are destroyed

24
Q

What is osmosis controlled in cells by which membrane

A

Cytoplasmic membrane

25
Q

Define osmosis

A

the diffusion of water from high water concentration (low solute concentration) to low water concentration (high solute concentration.

26
Q

Halophiles are _____ loving

A

NaCl loving

27
Q

Osmophiles can grow in high ____ concentration

A

Sugar concentrations

28
Q

What is commonly used as preservative to inhibit microbial growth

A

•salt
•sugar

29
Q

How do microbes grow under condition of low water availability

A

High NaCl
Low H2O availability
- increase internal solute concentration

30
Q

Compatible solutes are highly _____ soluble

A

Water soluble

31
Q

Microserophiles

A

Grow at reduced O2 concentrations

32
Q

Anaerobes

A

Cannot use oxygen for respiration

33
Q

Facultative anaerobes

A

Can grow in presence or absence of oxygen

34
Q

Some Gram positive bacteria can form _____ which provide protection from adverse conditions

A

Spores

35
Q

What is the spore in bacteria surrounded by

A

peptidoglycan-rich cortex layer and a keratin-like spore coat.

36
Q

Spores are resistant to

A

•Heat
•Drying,
•Radiation,
•Freezing,
•Toxic chemicals
•Antibiotics &
•Can be difficult to eradicate with standard disinfectants.

37
Q

Spores are involved in the transmission of what diseases

A

anthrax, caused by Bacillus anthracis

tetanus, caused by Clostridium tetani;

botulism, caused by Clostridium botulinum

*gas gangrene, caused by Clostridium perfringens

38
Q

Treatment for B.anthracis- Anthrax

A

•Penicillin
•Ciprofloxacin

39
Q

Anthrax symptoms

A

High fever

Massive swelling

Death

40
Q

Coagulase-negative staphylococci form

A

biofilms or slime on implanted biomaterials and catheters

41
Q

Biofilms form when bacteria adhere to

A

surfaces and excrete
slimy glue-like substances which anchor the cells

42
Q

What are Biofilm resistant to

A

Antimicrobial agents