Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

microbiology > Lecture 5 Microbial Growth And Physiology > Flashcards

Lecture 5 Microbial Growth And Physiology Flashcards

1
Q

Boiling is what temperature and how long

A

•100 degrees celcius
•30 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Autoclave is at what temperature and for how long

A

120 degrees celcius
30 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Dry heat is at what temperature and for how long

A

•150 degrees celcius
•120 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What process do bacteria divide by

A

Binary fission

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

When the chromosome divides during binary fission what does it produce

A

Two identical copies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Is Bacterial growth described as exponential

A

Yes the generation time (time for bacterial mass to double) can be as fast as 20 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What temperature does agar melt as

A

100 degrees celcius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What temperature does agar solidify at

A

40 degrees celcius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How is Agar sterilised

A

By Autoclaving

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What do bacteria grow as on agar

A

As Colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

How many cells/ml for growth in a liquid medium

A

10^9 cells/ml

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

For growth in a liquid medium what is position for incubation

A

Standing or Agitated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are the growth phases of a Bacterial Culture

A

•Lag
•Log
•Stationary
•Death

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Describe each of the growth phases of a Bacterial Culture

A

•Lag
Adaption
•Log
Cells multiply at the maximum rate
•Stationary
Lack of nutrients and build up of toxic metabolic intermediates means multiplication is balanced by cell death
•Death

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Describe the two direct measurements of Bacterial growth

A

•Total Cell Count
Using microscope and counting chamber

•Total Viable Count
Cells in culture are diluted and spread on nutrient agar plates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Total viable count is a serial ___ -fold dilution

A

10

17
Q

Name the indirect measurement of bacterial growth

A

•Turbidity
•Cloudiness
•Measure live and dead cells

18
Q

What is the nm of a filter or prism in a Spectrophotometer

A

•540nm

19
Q

In a Chemostat culture what is cell density controlled by

A

Nutrient Concentration

20
Q

What is Growth rate controlled by in a Chemostat culture

A

By the flow rate of nutrient

21
Q

What does Growth require

A

•Energy
•Building blocks required for construction of cellular machinery
•Appropriate environmental conditions

22
Q

Name the nutrient requirements for Growth

A

– Water
– Carbon (carbohydrate)
– Nitrogen (protein)
– Inorganic salts Iron - siderophores – Oxidation of organic compounds
– (carbohydrates, lipids, proteins)

23
Q

What temperature is required for Growth

A

20-110 degrees celcius

24
Q

What pH is required for Growth

A

4.0-9.0

25
Q

What type of Atmosphere for growth

A

O2/ No O2

26
Q

What is Energy derived from

A

The enzymatic breakdown of organic substrates (carbohydrates,lipids or proteins) in a process called CATOBOLISM

27
Q

What is Energy generated from CATABOLISM used to synthesise

A

Cellular constituents in a process called ANABOLISM

28
Q

Catabolism + Anabolism =

A

Metabolism

29
Q

Other than carbohydrates,lipids and proteins where else can bacteria derive energy from

A

•Plastic
•Rubber
•Toxic compounds like Phenol

30
Q

What are Auxotrophs

A

The ability of individual bacterial species to produce their own cellular components will dictate its nutritional requirement

31
Q

Obligate Aerobes grow only in the presence of what

A

O2

32
Q

Obligate Anaerobes grown only in the absence and are killed by

A

O2

33
Q

Facultative Anaerobes grow in the presence and absence and of

A

O2

34
Q

What are Psychrophile bacteria

A

•Cold loving bacteria
•10-20 degrees Celsius

35
Q

What are Mesophile bacteria

A

•Human body temperature
•Pathogens
•Opportunists
•20-40 degrees Celsius

36
Q

What are Thermophile Bacteria

A

•Heat loving
•>60 degrees Celsius

37
Q

Some bacteria can grow / survive in what ph

A

•acid
•alkali

microbiology (18 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions