Lecture 15 How To Identify Microorganisns

Question 1

What is used to produce an anaerobic environment for bacteria

Answer 1

Microaerophilic jars

Question 2

Name an example of a selective and differential agar

Answer 2

Eosin Methylene Blue

Question 3

Describe Eosin Methylene blue Agar

Answer 3

•Isolation of gram negative microorganism
•Lactose + Sucrose - no glucose

Question 4

Describe appearence of E. coli colonies on Eosin methylene blue agar

Answer 4

Black colonies with greenish sheen

Question 5

Describe Salmonella on Eosin methylene blue agar

Answer 5

Translucent Pink

Question 6

Biochemical tests in media look for

Answer 6

•Ability to ferment sugars
•Gas production (In agar- agar is pushed up) (In liquid - it’s collected in vials)

Question 7

Is E.coli oxidase negative or positive

Answer 7

Oxidase negative

Question 8

Is Pseudomonas oxidase negative or positive

Answer 8

Positive

Question 9

Name the biochemical test

Answer 9

Analytical profile index

Question 10

Name the types of Immunological tests

Answer 10

•Neutralisation test
•Viral Hemagglutination test
•Fluorescent Igs
•Enzyme linked Immunosorbent assay(ELISA)
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