Lecture 6

Question 1

What is sequencing

Answer 1

Automated process to determine the base pair sequence of nucleic acids

Question 2

What is metagenomics

Answer 2

Sequence everything in a sample

Question 3

What are the antibodies stained with

Answer 3

Antibodies conjugated to fluorochromes

Question 4

What is immunochromatography

Answer 4

An absorbent strip adhered to a card has a nitrocellulose during manufacture

Question 5

What does ELISA stand for

Answer 5

Enzyme linked immunosorbent assay

Question 6

What is the specificity of ELISA dependent on

Answer 6

Specificity of the capture antibody

Question 7

What is PRC

Answer 7

Amplifying technique for DNA

Question 8

What are the two assays of real time PCR

Answer 8

1. SybrGreen assay

2. Taqman assay

Question 9

What can the serum viral neutralising test be used for

Answer 9

Sterotyping of viruses

Question 10

What does ELISA stand fo

Answer 10

Enzyme linked immunosorbent assay

Question 11

What does ELISA test for

Answer 11

Antigen and antibody testing

Question 12

What is a capture antibody ELISA

Answer 12

Specificity of the assay is largely dependent on the specificity of the capture body

Question 13

The evaluation of assays (5)

Answer 13

1. Sensitivity
2. Specificity
3. Convenience
4. Cost
5. Repeatability