Lecture 5

Question 1

What is antigen detection

Answer 1

Demonstrate the organism

Question 2

What is antibody detection

Answer 2

Indirect evidence of presence of organism

Question 3

How to collect samples (2)

Answer 3

1. Smears

2. Can extract nucleic acids from smears and histological sections and do PCR

Question 4

How must blood be collected

Answer 4

Aseptically

Question 5

How to pack blood

Answer 5

1. Primary receptacle
2. Protective 2nd layer
3. Rigid outer packaging

Question 6

What is a virus culture

Answer 6

Growth of live virus

Question 7

What is serology

Answer 7

Indirect presence of virus - reaction of immune system - antibody

Question 8

What are laboratory animas

Answer 8

Different animals are used for different microbes

Question 9

What do embryonate chicken eggs help with

Answer 9

Different routes of infection - fast dividing

Question 10

What are cell cultures

Answer 10

In vitro growth of animal/human cells

Question 11

What are TCID50

Answer 11

Statistical value indicating the infectious nature of a virus. 50% tissue culture infected dose.

Question 12

What is blood collected and put into (for virus isolation)

Answer 12

10ml in green-topped (heparin)

Question 13

What is blood collected and put into (for serology isolation)

Answer 13

Collect in 10m in red-topped (plain)

Question 14

What are signs cytopathic effect (4)

Answer 14

1. Degeneration
2. Syncytium
3. Inclusion bodies
4. Transformation

Question 15

What is syncytium

Answer 15

A few host cells which merge cells