Lecture 4B - Culture Media, Biosafety, and Aseptic Technique Flashcards
How to prepare media
- calculate how much you need
- prepare all the materials and weigh needed amount
- mix components acc. to specific medium
- sterilize acc. to standard conditions
- post-sterilization
what is the standard condition for sterilizing
121C
15-20 min.
15 psi
tending to absorb moisture from the air
hygroscopic
the containment principles, technologies, ad practices that are implemented to prevent unintentional exposure to pathogens and toxins, or their accidental release
biosafety
protection of microbiological assests from theft, loss or diversion, which could lead to the inappropriate use of these agents to cause public health harm
biosecurity
difference between biosafety and biosecurity
biosafety: managing risks caused by ACCIDENTAL exposure/release of pathogen
biosecurity: managing risks caused by INTENTIONAL exposure/release of pathogen
biosafety
keep people from bad bags
biosecurity
keep bad bugs from bad people
biosafety and biosecurity
what for?
protect
- users
- those outside the laboratory
- the environment
biosafety and biosecurity
how?
- practice and procedures
- safety equipment
- facility design and construction
- increasing levels of protection
biosafety + biosecurity = ?
biorisk
source or object (not action) that can cause harm in security terms (biosafety)
hazard
associated with a person who has intent to cause harm to other people, animals, or the institution (biosecurity)
threat
can be based on either a hazard, or a hazard and a threat
risk
a hazard can pose a __ in a given situation
risk
probability or chance that a particular adverse event due to presence of a hazard under a certain situation will occur
biorisk
biorisk = ? + ?
likelihood + consequence
(of occurance of an event)