Lecture 4B - Culture Media, Biosafety, and Aseptic Technique Flashcards

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1
Q

How to prepare media

A
  1. calculate how much you need
  2. prepare all the materials and weigh needed amount
  3. mix components acc. to specific medium
  4. sterilize acc. to standard conditions
  5. post-sterilization
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2
Q

what is the standard condition for sterilizing

A

121C
15-20 min.
15 psi

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3
Q

tending to absorb moisture from the air

A

hygroscopic

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4
Q

the containment principles, technologies, ad practices that are implemented to prevent unintentional exposure to pathogens and toxins, or their accidental release

A

biosafety

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5
Q

protection of microbiological assests from theft, loss or diversion, which could lead to the inappropriate use of these agents to cause public health harm

A

biosecurity

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6
Q

difference between biosafety and biosecurity

A

biosafety: managing risks caused by ACCIDENTAL exposure/release of pathogen

biosecurity: managing risks caused by INTENTIONAL exposure/release of pathogen

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7
Q

biosafety

A

keep people from bad bags

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8
Q

biosecurity

A

keep bad bugs from bad people

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9
Q

biosafety and biosecurity
what for?

A

protect
- users
- those outside the laboratory
- the environment

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10
Q

biosafety and biosecurity
how?

A
  1. practice and procedures
  2. safety equipment
  3. facility design and construction
  4. increasing levels of protection
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11
Q

biosafety + biosecurity = ?

A

biorisk

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12
Q

source or object (not action) that can cause harm in security terms (biosafety)

A

hazard

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13
Q

associated with a person who has intent to cause harm to other people, animals, or the institution (biosecurity)

A

threat

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14
Q

can be based on either a hazard, or a hazard and a threat

A

risk

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15
Q

a hazard can pose a __ in a given situation

A

risk

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16
Q

probability or chance that a particular adverse event due to presence of a hazard under a certain situation will occur

A

biorisk

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17
Q

biorisk = ? + ?

A

likelihood + consequence
(of occurance of an event)

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18
Q

the likelihood of an occurence determine if the risk is what

A
  • low
  • medium
  • high or
  • extremely high
19
Q
  • likelihood of infection and exposure via an infectour route of a biological agent
  • consequences of disease to the at risk population
A

biosafety

20
Q

likelihood of theft of a biological agent and the severity of the consequences of an attack with that agent

A

biosecurity

21
Q

Who are the populations of concern

A
  1. persons in and around the laboratory
  2. human population outside the laboratory
  3. animal community outside the laboratory
22
Q
  • identify and reduce risks before they evolve into near misses or incident
  • provides the highest practical protectiona nd the lowest practical exposure
A

biorisk management

23
Q

biorisk management provides what

A
  • highest practical protections
  • lowest practical exposure
24
Q

Biorisk management

A
  1. assessment
  2. mitigation
  3. performance
25
Q

laboratory biorisk management system

A
  1. biohazard identification (ex. cultures)
  2. biorisk assessment
  3. biorisk management
  4. biorisk communication
26
Q

biorisk assessment

A
  1. what can happen?
  2. what is the chance that it will happen? (likelihood)
  3. if it happens, what are the consequences?
27
Q

the graver the consequence, the __ the biorisk

A

higher

28
Q
  • high number of COVID-19 patients in hospital
  • no isolation ward
  • limited PPE
A

very high risk of spreading infection

29
Q
  • high number of COVID-19 patients in a hospital
  • with isolation ward
  • continuous supply of PPEs
A

moderate risk of spreading infection

30
Q
  • low number of COVID-19 patients in a hospital
  • with isolation ward
  • continuous supply of PPEs
A

very low risk of spreading infection

31
Q

a hazard cannot pose a risk without a __ __

A

specific situation

32
Q

a situation does not represent a risk without a __

A

hazard

33
Q

a change in the _____ can change both __ & __

A
  • nature of hazard
  • likelihood
  • consequences
34
Q

can only change the likelihood but not the consequences

A

change in situation

35
Q

risk evaluation is a process determining __ whether a risk is high or low, and whether it is acceptable or not

A

subjectively

36
Q

highly influenced by the culture, experience, resources, managemen and even current events in the insitution and community

A

risk acceptance

37
Q

what highly influence risk acceptance

A
  1. culture
  2. experience
  3. resources
  4. management
  5. current events
38
Q

generally do not include perceived social, cultural, political, and ethical concerns

A

technical risk assessments

39
Q

risk acceptance will depend on the ‘owner’ of the risk

A
  1. risk averse
  2. risk tolerant
40
Q

what does risk evaluation drive in an institution

A

investment decisions

41
Q

will not spend a lot of resources

A

risk-tolerant individuals/institutions

42
Q

will more likely to spend resources to attempt reduce the risk they face

A

risk-averse individuals/institutions

43
Q

Benefits of a Robust Risk Assessment

A
  1. facilitates risk assessment process; repeatable/reproducible
  2. facilitates risk mitigation decisions
  3. provides quality control documentation