Lecture 3.3: Bacterial Genetics 3 Flashcards

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1
Q

the cell must regulate how much ___ protein it makes, as too much gives ___

A

rod(FlgE); polyhooks

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2
Q

Flik mutants can make

A

polyhooks

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3
Q

Flik is known as the __- because it acts as

A

hook length determinant because it acts as a ruler to give best length

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4
Q

when the hook is an optimal length, __ is secreted and __ are added

A

`Flik; capping protein

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5
Q

flik acts in the basal body as a

A

sensor for hook formation

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6
Q

it is believed that some flik remains in the basal body to

A

mediate other regulatory roles

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7
Q

the flagellar apparatus is modified to accumulate different __ at key points

A

substrates

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8
Q

what is FlhB?

A

membrane embedded protein in the basal body

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9
Q

FlhB autocleaves at the ___ after rod completion causes secretion of ___

A

c-terminal end; filament type proteins

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10
Q

what is Flit?

A

a dual post-translational regulatory system

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11
Q

what is Flid?

A

pentameric filament capping protein

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12
Q

where is the FliD?

A

on top of the rod after it is completed

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13
Q

Flit interacts with which protein?

A

FliD

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14
Q

Flid serves to facilitate ___ into the filament

A

flagellin protein incorporation

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15
Q

Flit helps __ Flid into cytoplasm for its export to the __

A

stabalize, rod tip

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16
Q

what is the role of uncomplexed Flit?

A

represses class 2 genes expression so class 3 can be expressed

17
Q

what is the rod protein?

A

FlgE

18
Q

post-translational regulatory control occurs when proteins __

A

have regulatory role after theyre made

19
Q

how does the cell sort through many different types of proteins?

A

flexibility through substrate switching

20
Q

two examples of post-translational regulatory proteins

A

FlhB and FliT

21
Q

once FlhB cuts itself, only __ are accepted

A

filament type

22
Q

FlhB has 2 modes of operation….why?

A

1 in cleaved form and one in uncleaved form

23
Q

Flit promotes filament formation in what 2 ways?

A

helping add Flid cap and by repressing class 2 genes