Lecture 3.3: Bacterial Genetics 3 Flashcards
the cell must regulate how much ___ protein it makes, as too much gives ___
rod(FlgE); polyhooks
Flik mutants can make
polyhooks
Flik is known as the __- because it acts as
hook length determinant because it acts as a ruler to give best length
when the hook is an optimal length, __ is secreted and __ are added
`Flik; capping protein
flik acts in the basal body as a
sensor for hook formation
it is believed that some flik remains in the basal body to
mediate other regulatory roles
the flagellar apparatus is modified to accumulate different __ at key points
substrates
what is FlhB?
membrane embedded protein in the basal body
FlhB autocleaves at the ___ after rod completion causes secretion of ___
c-terminal end; filament type proteins
what is Flit?
a dual post-translational regulatory system
what is Flid?
pentameric filament capping protein
where is the FliD?
on top of the rod after it is completed
Flit interacts with which protein?
FliD
Flid serves to facilitate ___ into the filament
flagellin protein incorporation
Flit helps __ Flid into cytoplasm for its export to the __
stabalize, rod tip
what is the role of uncomplexed Flit?
represses class 2 genes expression so class 3 can be expressed
what is the rod protein?
FlgE
post-translational regulatory control occurs when proteins __
have regulatory role after theyre made
how does the cell sort through many different types of proteins?
flexibility through substrate switching
two examples of post-translational regulatory proteins
FlhB and FliT
once FlhB cuts itself, only __ are accepted
filament type
FlhB has 2 modes of operation….why?
1 in cleaved form and one in uncleaved form
Flit promotes filament formation in what 2 ways?
helping add Flid cap and by repressing class 2 genes
