Lecture 23 Flashcards

1
Q

What happens when you put amphiphilic molecules in a non polar environment?

A

They tend to arrange themselves so that the nonpolar portions of their structure are away from interactions from aqueous solvents

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2
Q

What do Glycerophospholipids do when you place them in aqueous environments?

A

They tend to form bilayer structures like liposomes

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3
Q

What is a Liposome?

A

A small spherical structure that contains a bilayer of membrane lipids

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4
Q

What kind of environments do Liposomes create?

A

Distinct environments that are separate from each other. An interior and exterior environment. Inner leaflets and outer leaflets

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5
Q

Why is it important to form the distinction between inner leaflets and outer leaflets in liposomes?

A

Because in more complex structures it makes a difference weather it is in a cytoplasmic environment or exterior environment

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6
Q

How can liposomes be used for various different purposes?

A

Water soluble drugs can be placed in the center. Lipid soluble drugs can be placed in the bilayer portion

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7
Q

Why are Glycerophospholipids able to form phospholipids and triacylglycerols are not?

A
  • Glycerophospholipids are amphipathic and aggregate into bilayers that can interact with water on both sides
  • They also have to acyl chains that align easily to form the side by side to form a bilayer
  • Triacylglycerols are not amphipathic
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8
Q

What causes Bilayers to vary?

A
  • Acyl chain

* Polar head group

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9
Q

What kind of associations are formed between one lipid molecule in a bilayer and another?

A

Non-covalent associations

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10
Q

What does the non-covalent interactions between the lipids in a bilayer allow for?

A

It allows for stability but fluidity of the membrane

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11
Q

What are the characteristics of a bilayer with short acyl chains?

A

The bilayer will be thinner

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12
Q

What do the OH groups of cholesterol associate with in the bilayer?

A

The polar headgroups

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13
Q

What happens to a bilayer below the transition temperature?

A
  • Acyl chains pack together in van der waals contact

* Bilayer is in a gel-like solid state

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14
Q

What happens to a bilayer above the transition temperature?

A

Lipid molecules and their acyl chains move freely and rapidly

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15
Q

What is the melting temperature (transition temperature) of a lipid bilayer?

A

The temperature of its transition from an ordered crystalline to a more fluid state, and depends on acyl-chain unsaturation and length

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16
Q

What does the transition temperature of a bilayer depend on?

A

The acyl-chain unsaturation and length

17
Q

What type of membranes have a sharp transition temperature?

A

Artificial membranes that have one type of lipid

18
Q

What is the transition temperature for biological membranes usually like?

A

It is not typically sharp

19
Q

Why is the transition state for not usually sharp?

A

•Because it is a mixture of different compounds (different lipids/proteins)

20
Q

How can a membrane maintain a fluid state at different temperatures?

A
  • Introduce double bonds

* Changing the length of fatty acids

21
Q

What changes will be seen in the bilayer with decreasing temperature and why?

A
  • More unsaturated fatty acids
  • Shorter fatty acids
  • To lower the melting point
22
Q

What changes will be seen in the bilayer with increasing temperature and why?

A
  • More saturated fatty acids
  • Longer fatty acids
  • To increase melting point
23
Q

What does Cholesterol do to membranes in mammals?

A

It increases membrane rigidity and fluidity

24
Q

How does Cholesterol limit the acyl cells?

A

Because it is rigid and planar it can sit between the two acyl tails and increase van der Waals interactions causing them to be rigid

25
Q

What does Cholesterol do at low and high temperatures?

A
  • Low temps: Prevents close packing between acyl chains

* High temps: Decreases motion/disorder of acyl chains, increases rigidity

26
Q

In which direction can lipids move freely in the bilayer?

A

Laterally

27
Q

Why can’t lipids move from one leaflet to another rapidly (transverse)?

A

Because it is energetically unfavorable

28
Q

What do Flipases do?

A

Increase the rate of transverse diffusion

29
Q

In what organisms is E.coli present?

A

It is present in mammals only

30
Q

What are the three types of membrane proteins?

A
  • Integral membrane proteins
  • Peripheral membrane protein
  • Lipid-linked protein
31
Q

Through what interactions do integral binding proteins associate with lipids?

A

Through hydrophobic interactions

32
Q

What is the phobicity of the portion of the integral membrane protein surface that interacts with the acyls?

A

It is hydrophobic

33
Q

How do peripheral membrane proteins interact with the lipid bilayer?

A

Through electrostatic interactions. Charge charge and H-bond

34
Q

What are Lipid-linked proteins?

A

Proteins that contain a lipid prosthetic group

35
Q

How do Lipid-linked proteins associate with the membrane?

A

The anchor is covalently attached to the polypeptides