lab quiz 2 Flashcards
we cannot use a microscope to see
viruses
resolution
when you can tell 2 objects apart
magnification
how big something is
relationship between magnification and field of vision
magnification goes up, field of vision goes down
why use immersion oil with the 100x objective lens?
oil helps generate more light at higher magnification, because its index of refraction bends light more directly into the lens
proper way to carry microscope?
unscrew ocular section and carry separately, carry the rest by the neck.
typical magnification of an ocular lens?
10x
scanning lens
4x; they provide observers with about enough magnification for a good overview of the slide, essentially a “scan” of the slide
low power lens
10x;
high power lens
40x; ideal for observing fine details within a specimen sample.
oil immersion lens
100x; Without adding a drop of immersion oil, the oil immersion objective lens will not function correctly, the specimen will appear blurry, and you will not achieve an ideal magnification or resolution.
types of objective lenses?
scanning, low power, high power, oil immersion
total magnification of a sample with an ocular lens power of 10x using the high power lens
10 x 40 = 400x
what is the diaphragm?
constricts to control how much light is let in
why start with low power scanning lenses?
easier to focus IN rather than having to backtrack out.