lab final 1 Flashcards

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1
Q

scientific method steps

A

questions, observations, hypotheses, testing, testing, conclusions, etc

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2
Q

dependent variable goes on __ axis

A

y

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3
Q

independent variable goes on __ axis

A

x

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4
Q

____ data is presented in a line figure

A

continuous

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5
Q

___ data is presented in a histogram or bar graph

A

discrete

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6
Q

where does a figure description go?

A

under the figure
it should have enough information where a reader can understand the figure just by reading the description

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7
Q

introduction section:

A

-background
-purpose
-hypothesis

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8
Q

methods section:

A

-basic procedures
-important changes in procedures

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9
Q

results section:

A

-tables/figures AND headings
-written description

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10
Q

discussion section

A

-do results match hypothesis
_meaning of hypothesis
-possible errors
-new hypothesis
-major conclusions

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11
Q

function of a spectrophotometer?

A

A spectrophotometer is an instrument that measures the amount of photons (the intensity of light) absorbed after it passes through sample solution. With the spectrophotometer, the amount of a known chemical substance (concentrations) can also be determined by measuring the intensity of light detected.

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12
Q

absorbance vs transmittance?

A

Transmittance refers to the amount of light that passes completely through the sample and strikes the detector. Absorbance is a measurement of light that is absorbed by the sample.

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13
Q

what is a “blank”?

A

A blank is a sample that contains everything except for the analyte of interest.

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14
Q

what are enzymes are why are they important?

A

enzymes are biological catalyst that speed up chemical reactions by reducing activation energy.

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15
Q

What are enzyme substrates?

A

Enzymes are proteins that act upon substrate molecules and decrease the activation energy necessary for a chemical reaction to occur by stabilizing the transition state.

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16
Q

what are enzyme products?

A

The substrate goes through a chemical reaction and changes into a new molecule called the product

17
Q

what are enzyme activators?

A

chemicals that bind to an enzyme to increase its activity

18
Q

what are enzyme inhibitors?

A

chemicals that bind to an enzyme to decrease its activity

19
Q

how do we measure the rate at which an enzyme works?

A

determine the rate at which substrate is used OR determine the rate at which product is formed

20
Q

Why do some fruits turn brown when they are cut?

A

The substrates react with one another within the active site of the enzyme. The products formed by this reaction are benzoquinone and water; since benzoquinone has a brown color, you can see that the reaction has taken place. This is called the fruit browning reaction.

21
Q

What are Catechol Oxidase, Catechol, and Benzoquinone?

A

catechol oxidase: enzyme
catechol: substrate
benzoquinone: product

22
Q

How does TLC work?

A

thin layer chromatography separates pigments from each other n
on-polar compounds move up the plate most rapidly , whereas polar substances travel up the TLC plate slowly or not at all.

23
Q

mobile phase vs stationary phase

A

The silica gel is the stationary phase. The mobile phase is a suitable liquid solvent or mixture of solvents.

24
Q
  • Why do scientists repeat experiments?
A

lets you see patterns and trends in your results

25
Q
  • What is a Solute? What is a Solvent?
A

The solute is the substance that is being dissolved, while the solvent is the dissolving medium

26
Q

osmosis

A

osmosis is the movement of water molecules from a solution with a high concentration of water molecules to a solution with a lower concentration of water molecules, through a cell’s partially permeable membrane.

27
Q

hypertonic

A

more solute in solution (water leaves cell)

28
Q

hypotonic

A

less solutes in solution (water enters cell and it swells)

29
Q

isotonic

A

equal solute concentration

30
Q
  • How does dialysis tubing work?
A

dialysis tubing (membrane of tightly packed cellulose fibers) has pores that let certain molecules to pass through

31
Q

What is a semi-permeable membrane?

A

a membrane that will allow certain molecules or ions to pass through it by diffusion and occasionally specialized “facilitated diffusion”.

32
Q
  • In the osmosis/dialysis tubing experiment, why did you obtain the results you obtained?
A
33
Q

What is the physiological reason(s) why students in the “water”, “Gatorade”, and “control” groups had different urinary outputs?

A
34
Q
  • What are the functions of nephrons, Antidiuretic Hormone (Vasopressin), RAAS in your kidneys?
A
35
Q
  • How might “dialysis” be related to human health? What are some major causes of human kidney failure, and how does kidney dialysis work to overcome this kidney failure?
A