lab quiz 1 Flashcards
1
Q
mouse handling
A
- pay attention to which cage they came from
- handled with gloves to prevent allergies
- disinfect gloved hands between each cage
- firm but gentle
- consistent (get used to same thing)
- respect
2
Q
mouse anatomy
A
- stomach
- Peyers patches on intestine
- seminal vesicles
- coagulating gland
3
Q
mouse identification methods
A
ear punch
tattoo
ear clip
4
Q
physical exam
A
temp: 98
pulse: 320-780
resp: 84-280 (180 normal)
abnormalities: odd gait or behavior, ocular or nasal discharge, skin problems, diarrhea, weight loss, dental issues
5
Q
how much blood can you take from a mouse
A
max of 1% of weight in grams (wt x 0.01 = mL)
6
Q
bleeding sites
A
- intracardiac
- retro-orbital
- facial or superficial temporal vein
- tail (lateral tail vein or ventral tail artery)
- lateral saphenous vein
7
Q
how to administer medicines to mice
A
- gavage: by mouth to stomach
- SQ injections: 22 g needle no more; 2 mL max
- IM injections: 25 g needle; 0.05 mL max
- IV lateral tail vein: heat mouse, don’t aspirate
- IP injection: 25 g; 2 mL max; angle down; lower right quadrant
8
Q
mouse behavior
A
- crepuscular
- polygamous (males individually and females in groups)
- timid, gentle, quick
9
Q
mouse restraint
A
pick up by tail - scruff whole body - and can use restraining devices (squeeze tubes and bags)
