lab 5 Flashcards
1
Q
what areas did PCR have an impact on
A
- gene mapping
- cloning
- DNA sequencing
- gene detection
2
Q
PCR template
A
any form of ds DNA
3
Q
PCR ingredients
A
- reaction buffer
- DNA subunits
- DNA polymerase
- 2 DNA primers
- template strand
4
Q
what 2 basic processes does PCR make use of
A
- complementary DNA strand hybridization
- DNA strand synthesis via DNA polymerase
5
Q
DNA polymerase used
A
Taq polymerase from Thermus aquaticus
6
Q
steps of PCR
A
- denaturation
- primer annealing
- primer extension
7
Q
term for PCR machine
A
thermal cycler
8
Q
temperature/thermal cycling
A
rapid heating and cooling of thermal block in thermal cycler
9
Q
temp at denaturation step
A
94 degrees Celsius
10
Q
temp at annealing step
A
60 degrees Celsius
11
Q
temp at extension step
A
72 degrees Celsius
12
Q
temperature cycle =
A
denaturation + annealing + extension
13
Q
PCR generates DNA of a…
A
precise length and sequence
14
Q
which cycle are fragments of precise length generated
A
third
15
Q
what are targets of random insertions?
A
introns
