lab 4

Question 1

restriction fragment length polymorphism

Answer 1

cut DNA to look at length, then look at how they are unique among indivduals

Question 2

what kind of enzymes are restriction enzymes

Answer 2

endonucleases

Question 3

what DNA are we using today that is the same as last week

Answer 3

EcoRI, PstI

Question 4

what are inverted repeats also known as?

Answer 4

palindromes

Question 5

where are restriction enzymes found?

Answer 5

bacterial defense systems

Question 6

how can the DNA that has been cut be separated and observed

Answer 6

agarose gel electrophoresis

Question 7

recognition sequence

Answer 7

4-6 bp segment of DNA that restriction enzymes recognizes

Question 8

2 commonly used enzymes in this lab

Answer 8

EcoRi and PstI

Question 9

what DNA stain are we using

Answer 9

UView

Question 10

components of UView

Answer 10

• bromphenol blue
• xylene cylanol
• fluorescent stain

Question 11

what DNA standard are we using

Answer 11

1 kb DNA Ladder

Question 12

2 major factors affecting reliability of DNA fingerprinting technology

Answer 12

1. population genetics
2. genetic statistics

Question 13

plasmid

Answer 13

circular, non-chromosomal pieces of DNA

Question 14

transformation

Answer 14

procedure in which genetically engineered DNA sequence is inserted into plasmid and then reinserted back into bacterial cells

Question 15

cloned DNA

Answer 15

foreign piece of DNA on plasmid

Question 16

vector

Answer 16

plasmid that is carrying the foreign DNA

Question 17

type of plasmid used

Answer 17

pTZ18U

Question 18

what are ends called where restriction enzyme has cut

Answer 18

sticky ends